SCI和EI收录∣中国化工学会会刊

›› 2011, Vol. 19 ›› Issue (6): 1039-1046.

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Supercritical Antisolvent-based Technology for Preparation of Vitamin D3 Proliposome and Its Characteristics

夏菲1, 金鹤阳1, 赵亚平1, 郭新秋2   

  1. 1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
    2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China
  • 收稿日期:2010-12-07 修回日期:2011-07-28 出版日期:2011-12-28 发布日期:2012-04-24
  • 通讯作者: ZHAO Yaping, E-mail: ypzhao@sjtu.edu.cn
  • 基金资助:
    Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

Supercritical Antisolvent-based Technology for Preparation of Vitamin D3 Proliposome and Its Characteristics

XIA Fei1, JIN Heyang1, ZHAO Yaping1, GUO Xinqiu2   

  1. 1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
    2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2010-12-07 Revised:2011-07-28 Online:2011-12-28 Published:2012-04-24
  • Supported by:
    Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

摘要: Vitamin D3 (VD3) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD3, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD3 loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD3 and HPC, the VD3 loading reached 12.89%. VD3 liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD3 in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD3 nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

关键词: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent

Abstract: Vitamin D3 (VD3) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD3, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD3 loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD3 and HPC, the VD3 loading reached 12.89%. VD3 liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD3 in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD3 nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

Key words: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent