Supercritical Antisolvent-based Technology for Preparation of Vitamin D3 Proliposome and Its Characteristics

›› 2011, Vol. 19 ›› Issue (6): 1039-1046.

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

夏菲¹, 金鹤阳¹, 赵亚平¹, 郭新秋²

1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China

收稿日期:2010-12-07 修回日期:2011-07-28 出版日期:2011-12-28 发布日期:2012-04-24
通讯作者: ZHAO Yaping, E-mail: ypzhao@sjtu.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

XIA Fei¹, JIN Heyang¹, ZHAO Yaping¹, GUO Xinqiu²

1. College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai 200240, China

Received:2010-12-07 Revised:2011-07-28 Online:2011-12-28 Published:2012-04-24
Supported by:
Supported by the National High Technology Research and Development Program of China (2007AA10Z350);the National Natural Science Foundation of China (20976103)

摘要/Abstract

摘要： Vitamin D₃ (VD₃) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD₃, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD₃ loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD₃ and HPC, the VD₃ loading reached 12.89%. VD₃ liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD₃ in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD₃ nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

关键词: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent

Abstract: Vitamin D₃ (VD₃) proliposomes (VDP), consisted of hydrogenated phosphatidycholine (HPC) and VD₃, were prepared using supercritical anti-solvent technology (SAS). The effects of operation conditions (temperature, pressure and components) on the VD₃ loading in VDP were studied. At the optimum conditions of pressure of 8.0 MPa, temperature of 45℃, and the mass ratio of 15.0% between VD₃ and HPC, the VD₃ loading reached 12.89%. VD₃ liposomes (VDL) were obtained by hydrating VDP and the entrapment efficiency of VD₃ in VDL reached 98.5%. The morphology and structure of VDP and VDL were characterized by SEM (scanning electron microscope), TEM (transmission electron microscope) and XRD (X-ray diffractometer). The structure of VD₃ nanoparticles in HPC matrix was formed. The size of VDL with an average diameter of about 1μm was determined by dynamic light scattering instrument (DLS). The results indicated that VDP can be made by SAS and VDL with high entrapment efficiency can be formed easily via the hydration of VDP.

Key words: hydrogenated phosphatidycholine, liposome, encapsulation, supercritical anti-solvent

夏菲, 金鹤阳, 赵亚平, 郭新秋. Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics[J]. , 2011, 19(6): 1039-1046.

XIA Fei, JIN Heyang, ZHAO Yaping, GUO Xinqiu. Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics[J]. , 2011, 19(6): 1039-1046.

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Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

Supercritical Antisolvent-based Technology for Preparation of Vitamin D₃ Proliposome and Its Characteristics

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