n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

doi:10.1016/j.cjche.2018.03.034

Chinese Journal of Chemical Engineering ›› 2019, Vol. 27 ›› Issue (2): 411-417.DOI: 10.1016/j.cjche.2018.03.034

• Biotechnology and Bioengineering • 上一篇下一篇

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

Xiaoqiang Jia^1,2,3, Yun He¹, Lei Huang¹, Dawei Jiang¹, Wenyu Lu^1,2,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
3 Synthetic Biology Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

收稿日期:2018-01-11 修回日期:2018-03-23 出版日期:2019-02-28 发布日期:2019-03-18
通讯作者: Wenyu Lu
基金资助:
Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), and Tianjin Key Research & Development Program (16YFXTSF00460)

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

Xiaoqiang Jia^1,2,3, Yun He¹, Lei Huang¹, Dawei Jiang¹, Wenyu Lu^1,2,3

1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
2 Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
3 Synthetic Biology Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China

Received:2018-01-11 Revised:2018-03-23 Online:2019-02-28 Published:2019-03-18
Contact: Wenyu Lu
Supported by:
Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), and Tianjin Key Research & Development Program (16YFXTSF00460)

摘要/Abstract

摘要： The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n-hexadecane are both difficult to degrade. In this study, n-hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n-hexadecane (2 vol%) and 42.79% pyrene (200 mg·L^-1) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n-hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35℃, 0.5% NaCl, 5 mg·L^-1 of yeast extract and 90 mg·L^-1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.

关键词: Biodegradation, Metabolite, n-Hexadecane, Pyrene, Rhodococcus sp.T1

Abstract: The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n-hexadecane are both difficult to degrade. In this study, n-hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n-hexadecane (2 vol%) and 42.79% pyrene (200 mg·L^-1) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n-hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35℃, 0.5% NaCl, 5 mg·L^-1 of yeast extract and 90 mg·L^-1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.

Key words: Biodegradation, Metabolite, n-Hexadecane, Pyrene, Rhodococcus sp.T1

Xiaoqiang Jia, Yun He, Lei Huang, Dawei Jiang, Wenyu Lu. n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil[J]. Chinese Journal of Chemical Engineering, 2019, 27(2): 411-417.

参考文献

[1] G.A. Silvacastro, B. Rodelas, C. Perucha, J. Laguna, J. Gonzalezlopez, C. Calvo, Bioremediation of diesel-polluted soil using biostimulation as post-treatment after oxidation with Fenton-like reagents:assays in a pilot plant, Sci. Total Environ. 347(2013) 445-446.

[2] Y. Chen, C. Li, Z. Zhou, J. Wen, X. You, Y. Mao, C. Lu, G. Huo, X. Jia, Enhanced biodegradation of alkane hydrocarbons and crude oil by mixed strains and bacterial community analysis, Appl. Biochem. Biotechnol. 172(2014) 3433-3447.

[3] L. Meng, H. Li, M. Bao, P. Sun, Metabolic pathway for a new strain Pseudomonas synxantha LSH-7:from chemotaxis to uptake of n-hexadecane, Sci. Rep. 7(2017) 39068.

[4] Z. Wang, Z. Liu, Y. Yang, T. Li, M. Liu, Distribution of PAHs in tissues of wetland plants and the surrounding sediments in the Chongming wetland, Shanghai, China, Chemosphere 89(2012) 221-227.

[5] D. Ollis, Slick solution for oil spills, Nature 358(1992) 453-454.

[6] L.B. Salam, O.S. Obayor, O.S. Akashoro, G.O. Okogie, Biodegradation of bonny light crude oil by bacteria isolated from contaminated soil, Int. J. Agric. Biol. 13(2011) 1560-853013.

[7] L.W. Peng, M.J. Sheu, L.Y. Lin, C.T. Wu, H.M. Chiang, W.H. Lin, M.C. Lee, H.C. Chen, Effect of heat treatments on the essential oils of kumquat (Fortunella margarita Swingle), Food Chem. 136(2013) 532-537.

[8] C. Okieimen, F. Okieimen, Effect of natural rubber processing sludge on the degradation of crude oil hydrocarbons in soil, Bioresour. Technol. 82(2002) 95-97.

[9] M.B. Yerima, A.A. Balogun, A.A. Farouq, S. Muhammad, Laboratory based degradation of light crude oil by aquatic phycomycetes, Afr. J. Biotechnol. 8(2009) 3851-3853.

[10] M. Romantschuk, I. Sarand, T. Petanen, R. Peltola, M. Jonsson-Vihanne, T. Koivula, K. Yrjala, K. Haahtela, Means to improve the effect of in situ bioremediation of contaminated soil:an overview of novel approaches, Environ. Pollut. 107(2000) 179-185.

[11] L. Martinkova, B. Uhnakova, M. Patek, J. Nesvera, V. Kren, Biodegradation potential of the genus Rhodococcus, Environ. Int. 35(2009) 162.

[12] J. Yan, J. Wen, H. Li, S. Yang, Z. Hu, The biodegradation of phenol at high initial concentration by the yeast Candida tropicalis, Biochem. Eng. J. 24(2005) 243-247.

[13] A.A. Shah, A. Nawaz, L. Kanwal, F. Hasan, S. Khan, M. Badshah, Degradation of poly (ε-caprolactone) by a thermophilic bacterium Ralstonia sp. strain MRL-TL isolated from hot spring, Int. Biodeterior. Biodegrad. 98(2015) 35-42.

[14] U. Walter, M. Beyer, J. Klein, H.J. Rehm, Degradation of pyrene by Rhodococcus sp. UW1, Appl. Microbiol. Biotechnol. 34(1991) 671-676.

[15] B. Cao, K. Nagarajan, K.C. Loh, Biodegradation of aromatic compounds:current status and opportunities for biomolecular approaches, Appl. Microbiol. Biotechnol. 85(2009) 207-228.

[16] B. Mahanty, K. Pakshirajan, D.V. Venkata, Biodegradation of pyrene by Mycobacterium frederiksbergense in a two-phase partitioning bioreactor system, Bioresour. Technol. 99(2008) 2694-2698.

[17] W. Qin, F.Q. Fan, Y. Zhu, Y. Wang, X. Liu, A. Ding, J. Dou, Comparative proteomic analysis and characterization of benzo(a)pyrene removal by Microbacterium sp. strain M.CSW3 under denitrifying conditions, Bioprocess Biosyst. Eng. 40(12) (2017) 1825-1838.

[18] L. Wang, Y. Tang, S. Wang, R.L. Liu, M.Z. Liu, Y. Zhang, F.L. Liang, L. Feng, Isolation and characterization of a novel thermophilic Bacillus strain degrading long-chain n-alkanes, Extremophiles 10(2006) 347.

[19] W. Wang, B. Cai, Z. Shao, Oil degradation and biosurfactant production by the deep sea bacterium Dietzia maris As-13-3, Front. Microbiol. 5(2014) 711.

[20] J.S. Seo, Y.S. Keum, Q.X. Li, Bacterial degradation of aromatic compounds, Int. J. Environ. Res. Public Health 6(2009) 278.

[21] M.R. Viant, U. Sommer, Mass spectrometry based environmental metabolomics:a primer and review, Metabolomics 9(2013) 144-158.

[22] M.Z. Ding, J.S. Cheng, W.H. Xiao, B. Qiao, Y.J. Yuan, Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS, Metabolomics 5(2009) 229.

[23] X. Pan, H. Liu, J. Liu, C. Wang, J. Wen, Omics-based approaches reveal phospholipids remodeling of Rhizopus oryzae responding to furfural stress for fumaric acidproduction from xylose, Bioresour. Technol. 222(2016) 24-32.

[24] M. Eshelli, L. Harvey, R. Edrada-Ebel, B. Mcneil, Metabolomics of the bio-degradation process of aflatoxin B1 by actinomycetes at an initial pH of 6.0, Toxins 7(2015) 439.

[25] R.A. Scott, S.E. Lindow, Transcriptional control of quorum sensing and associated metabolic interactions in Pseudomonas syringae strain B728a, Mol. Microbiol. 99(2015) 1080-1098.

[26] W. Lu, X. Su, M.S. Klein, I.A. Lewis, O. Fiehn, J.D. Rabinowitz, Metabolite measurement:pitfalls to avoid and practices to follow, Annu. Rev. Biochem. 86(2017) 277.

[27] O.G. Brakstad, A.G.G. Lodeng, Microbial diversity during biodegradation of crude oil in seawater from the north sea, Microb. Ecol. 49(2005) 94-103.

[28] O.S. Obayori, S.A. Adebusoye, A.O. Adewale, G.O. Oyetibo, O.O. Oluyemi, R.A. Amokun, M.O. Ilori, Differential degradation of crude oil (Bonny Light) by four Pseudomonas strains, J. Environ. Sci. 21(2009) 243-248.

[29] A. Wentzel, H. Sletta, S. Consortium, T.E. Ellingsen, P. Bruheim, Intracellular metabolite pool changes in response to nutrient depletion induced metabolic switching in Streptomyces coelicolor, Meta 2(2012) 178-194.

[30] M. Xia, D. Huang, S. Li, J. Wen, X. Jia, Y. Chen, Enhanced FK506 production in Streptomyces tsukubaensis by rational feeding strategies based on comparative metabolic profiling analysis, Biotechnol. Bioeng. 110(2013) 2717-2730.

[31] M.Z. Ding, X. Zhou, Y.J. Yuan, Metabolome profiling reveals adaptive evolution of Saccharomyces cerevisiae during repeated vacuum fermentations, Metabolomics 6(2010) 42-55.

[32] L. Huang, C. Liu, Y. Liu, X. Jia, The composition analysis and preliminary cultivation optimization of a PHA-producing microbial consortium with xylose as a sole carbon source, Waste Manag. 52(2016) 77.

[33] J.B. van Beilen, E.G. Funhoff, Expanding the alkane oxygenase toolbox:new enzymes and applications, Curr. Opin. Biotechnol. 16(2005) 308-314.

[34] C. Muangchinda, A. Yamazoe, D. Polrit, H. Thoetkiattikul, W. Mhuantong, V. Champreda, O. Pinyakong, Biodegradation of high concentrations of mixed polycyclic aromatic hydrocarbons by indigenous bacteria from a river sediment:a microcosm study and bacterial community analysis, Environ. Sci. Pollut. Res. 24(2017) 4591-4602.

[35] B. Wang, Q. Lai, Z. Cui, T. Tan, Z. Shao, A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp. P1, Environ. Microbiol. 10(2008) 1948-1963.

[36] S. Dietmair, M.P. Hodson, L.E. Quek, N.E. Timmins, P. Chrysanthopoulos, S.S. Jacob, P. Gray, L.K. Nielsen, Metabolite profiling of CHO cells with different growth characteristics, Biotechnol. Bioeng. 109(2012) 1404-1414.

[37] B. Wang, J. Liu, H. Liu, D. Huang, J. Wen, Comparative metabolic profiling reveals the key role of amino acids metabolism in the rapamycin overproduction by Streptomyces hygroscopicus, J. Ind. Microbiol. Biotechnol. 42(2015) 949.

[38] Y. Liu, J. Liu, C. Li, J. Wen, R. Ban, X. Jia, Metabolic profiling analysis of the degradation of phenol and 4-chlorophenol by Pseudomonas sp. cbp1-3, Biochem. Eng. J. 90(2014) 316-323.

[39] N. Lu, D. Wei, X. Jiang, F. Chen, S. Yang, Fatty acids profiling and biomarker identification in snow slga Chlamydomonas sivalis by NaCl stress using GC/MS and multivariate statistical analysis, Anal. Lett. 45(2012) 1172-1183.

[40] M. Abouseoud, A. Yataghene, A. Amrane, R. Maachi, Effect of pH and salinity on the emulsifying capacity and naphthalene solubility of a biosurfactant produced by Pseudomonas fluorescens, J. Hazard. Mater. 180(2010) 131-136.

[41] C. Dorta, R. Cruz, P. de Oliva-Neto, D.J.C. Moura, Sugarcane molasses and yeast powder used in the fructooligosaccharides production by Aspergillus japonicus-FCL 119T and Aspergillus niger ATCC 20611, J. Ind. Microbiol. Biotechnol. 33(2006) 1003.

[42] C.N. Mulligan, R.N. Yong, B.F. Gibbs, Surfactant-enhanced remediation of contaminated soil:a review, Eng. Geol. 60(2001) 371-380.

[43] H. Yu, G.H. Huang, H.N. Xiao, L. Wang, W. Chen, Combined effects of DOM and biosurfactant enhanced biodegradation of polycylic armotic hydrocarbons (PAHs) in soil-water systems, Environ. Sci. Pollut. Res. 21(2014) 10536-10549.

[44] W.H. Noordman, J.H.J. Wachter, G.J.D. Boer, D.B. Janssen, The enhancement by surfactants of hexadecane degradation by Pseudomonas aeruginosa varies with substrate availability, J. Biotechnol. 94(2002) 195.

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	Chao Zhang, Youzhi Liu, Weizhou Jiao, Hongyan Shen, Xigang Yuan, Shengkun Jia. An optimization method for enhancement of gas–liquid mass transfer in a bubble column reactor based on the entropy generation extremum principle[J]. 中国化学工程学报, 2023, 53(1): 83-88.
[2]	Samra Basharat, Ziyang Huang, Mengyue Gong, Xueqin Lv, Aqsa Ahmed, Iftikhar Hussain, Jianghua Li, Guocheng Du, Long Liu. A review on current conventional and biotechnical approaches to enhance biosynthesis of steviol glycosides in Stevia rebaudiana[J]. 中国化学工程学报, 2021, 29(2): 92-104.
[3]	Qun Wang, Lanhui Jiang, Chengran Fang, Hongzhi Mao, Haifeng Zhuang. Transformation of phthalic acid diesters in an anaerobic/anoxic/oxic leachate treatment process[J]. 中国化学工程学报, 2020, 28(1): 249-253.
[4]	Lijuan Shen, Xu Yan, Lei Nie, Wenyan Xu, Shiwei Miao, Haibin Wang, H. Fai Poon, Haibin Qu. Chemometric identification of canonical metabolites linking critical process parameters to monoclonal antibody production during bioprocess development[J]. 中国化学工程学报, 2019, 27(5): 1171-1176.
[5]	Mohammad Mehdi Amin, Bijan Bina, Afshin Ebrahimi, Zeynab Yavari, Farzaneh Mohammadi, Somayeh Rahimi. The occurrence, fate, and distribution of natural and synthetic hormones in different types of wastewater treatment plants in Iran[J]. Chinese Journal of Chemical Engineering, 2018, 26(5): 1132-1139.
[6]	Mohammad Mehdi Amin, Bijan Bina, Karim Ebrahim, Zeynab Yavari, Farzaneh Mohammadi. Biodegradation of natural and synthetic estrogens in moving bed bioreactor[J]. Chinese Journal of Chemical Engineering, 2018, 26(2): 393-399.
[7]	Bijan Bina, Farzaneh Mohammadi, Mohammad Mehdi Amin, Hamid Reza Pourzamani, Zeynab Yavari. Determination of 4-nonylphenol and 4-tert-octylphenol compounds in various types of wastewater and their removal rates in different treatment processes in nine wastewater treatment plants of Iran[J]. Chinese Journal of Chemical Engineering, 2018, 26(1): 183-190.
[8]	Qilong Ge, Xiuping Yue, Guoying Wang. Simultaneous heterotrophic nitrification and aerobic denitrification at high initial phenol concentration by isolated bacterium Diaphorobacter sp. PD-7[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 835-841.
[9]	Xianli Shi, Li Deng, Fangfang Sun, Jieyu Liang, Xu Deng. Key factors governing alkaline pretreatment of waste activated sludge[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 842-846.
[10]	Zongkuan Liu, Lei Zhang, Yonghong Liu, Yanling He. Anaerobic biodegradation of RDX and HMX with different co-substrates[J]. Chinese Journal of Chemical Engineering, 2015, 23(4): 704-709.
[11]	陈慧英, 王明霞, 沈煜斌, 姚善泾. Optimization of Two-species Whole-cell Immobilization System Constructed with Marine-derived Fungi and Its Biological Degradation Ability[J]. Chinese Journal of Chemical Engineering, 2014, 22(2): 187-192.
[12]	於建明, 蔡文吉, 赵士良, 王艳, 陈建孟. Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions[J]. Chinese Journal of Chemical Engineering, 2013, 21(7): 781-786.
[13]	隋红, 李鑫钢. Modeling for Volatilization and Bioremediation of Toluene-contaminated Soil by Bioventing[J]. , 2011, 19(2): 340-348.
[14]	刘景明, 孙冬冬, 刘慧, 聂英斌, 朱志荣. Biodegradation Kinetics for Pre-treatment of Klebsiella pneumoniae Waste with Autothermal Thermophilic Aerobic Digestion[J]. , 2010, 18(6): 905-909.
[15]	赵月春, 易筱筠, 李明华, 刘露, 马伟娟. Biodegradation Kinetics of DDT in Soil under Different Environmental Conditions by Laccase Extract from White Rot Fungi[J]. , 2010, 18(3): 486-492.