SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (7): 781-786.DOI: 10.1016/S1004-9541(13)60536-4

• 生物技术与生物工程 • 上一篇    下一篇

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

於建明1,2, 蔡文吉1, 赵士良1, 王艳1, 陈建孟1   

  1. 1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China;
    2 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China
  • 收稿日期:2012-02-13 修回日期:2012-10-08 出版日期:2013-07-28 发布日期:2013-08-24
  • 通讯作者: CHEN Jianmeng
  • 基金资助:

    Supported by the National Natural Science Foundation of China (20977087, 20976165), Zhejiang Provincial Key Science and Technology Project of China (2011C13023), and Zhejiang Provincial Natural Science Foundation of China (Y5090155, Y5090054).

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

YU Jianming1,2, CAI Wenji1, ZHAO Shiliang1, WANG Yan1, CHEN Jianmeng 1   

  1. 1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China;
    2 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China
  • Received:2012-02-13 Revised:2012-10-08 Online:2013-07-28 Published:2013-08-24
  • Supported by:

    Supported by the National Natural Science Foundation of China (20977087, 20976165), Zhejiang Provincial Key Science and Technology Project of China (2011C13023), and Zhejiang Provincial Natural Science Foundation of China (Y5090155, Y5090054).

摘要: A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH2Cl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L-1 to 10 g·L-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g·L-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH2BrCl, C2H4Cl2, and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R2 0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g·L-1 to 60 g·L-1. The growing cells (in minimal salt medium) degraded approximately 50% of the CH2Cl2 within 24 h, whereas the resting cells (in physiological saline) degraded only 25% of the CH2Cl2 within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.

关键词: chlorinated hydrocarbons, biodegradation, Bacillus circulans WZ-12, salinity

Abstract: A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH2Cl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L-1 to 10 g·L-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g·L-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH2BrCl, C2H4Cl2, and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R2 0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g·L-1 to 60 g·L-1. The growing cells (in minimal salt medium) degraded approximately 50% of the CH2Cl2 within 24 h, whereas the resting cells (in physiological saline) degraded only 25% of the CH2Cl2 within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.

Key words: chlorinated hydrocarbons, biodegradation, Bacillus circulans WZ-12, salinity