Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

doi:10.1016/S1004-9541(13)60536-4

Chinese Journal of Chemical Engineering ›› 2013, Vol. 21 ›› Issue (7): 781-786.DOI: 10.1016/S1004-9541(13)60536-4

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

於建明^1,2, 蔡文吉¹, 赵士良¹, 王艳¹, 陈建孟¹

1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China;
2 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China

收稿日期:2012-02-13 修回日期:2012-10-08 出版日期:2013-07-28 发布日期:2013-08-24
通讯作者: CHEN Jianmeng
基金资助:
Supported by the National Natural Science Foundation of China (20977087, 20976165), Zhejiang Provincial Key Science and Technology Project of China (2011C13023), and Zhejiang Provincial Natural Science Foundation of China (Y5090155, Y5090054).

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

YU Jianming^1,2, CAI Wenji¹, ZHAO Shiliang¹, WANG Yan¹, CHEN Jianmeng ¹

1 College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China;
2 College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310032, China

Received:2012-02-13 Revised:2012-10-08 Online:2013-07-28 Published:2013-08-24
Supported by:
Supported by the National Natural Science Foundation of China (20977087, 20976165), Zhejiang Provincial Key Science and Technology Project of China (2011C13023), and Zhejiang Provincial Natural Science Foundation of China (Y5090155, Y5090054).

摘要/Abstract

摘要： A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH₂Cl₂ was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L^-1 to 10 g·L^-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g·L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH₂BrCl, C₂H₄Cl₂, and C₂H₂Cl₂ in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R² 0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g·L^-1 to 60 g·L^-1. The growing cells (in minimal salt medium) degraded approximately 50% of the CH₂Cl₂ within 24 h, whereas the resting cells (in physiological saline) degraded only 25% of the CH₂Cl₂ within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.

关键词: chlorinated hydrocarbons, biodegradation, Bacillus circulans WZ-12, salinity

Abstract: A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CH₂Cl₂ was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g·L^-1 to 10 g·L^-1, and the maximum degradation efficiency (85%) was achieved at NaCl concentration of 10 g·L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH₂BrCl, C₂H₄Cl₂, and C₂H₂Cl₂ in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine (GB), but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation (R² 0.85-0.94) was observed between the rate of biodegradation (V) and salt concentration from 5 g·L^-1 to 60 g·L^-1. The growing cells (in minimal salt medium) degraded approximately 50% of the CH₂Cl₂ within 24 h, whereas the resting cells (in physiological saline) degraded only 25% of the CH₂Cl₂ within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industrial effluents that contain chlorinated hydrocarbons with high salt concentrations.

Key words: chlorinated hydrocarbons, biodegradation, Bacillus circulans WZ-12, salinity

於建明, 蔡文吉, 赵士良, 王艳, 陈建孟. Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions[J]. Chinese Journal of Chemical Engineering, 2013, 21(7): 781-786.

YU Jianming, CAI Wenji, ZHAO Shiliang, WANG Yan, CHEN Jianmeng . Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions[J]. Chin.J.Chem.Eng., 2013, 21(7): 781-786.

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Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

Aerobic Biodegradation of Chlorinated Hydrocarbons by Bacillus circulans WZ-12 CCTCC M 207006 under Saline Conditions

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