SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2024, Vol. 72 ›› Issue (8): 220-225.DOI: 10.1016/j.cjche.2024.06.002

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Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Yucong Gong1, Xiangli Li1, Daqing Ma2, Lai Wang1, Lin Zhou1, Caiwei Lu1, Yi Xiao1, Xinfu Zhang1   

  1. 1 State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
    2 China Academy of Safety Science and Technology, Beijing 100012, China
  • 收稿日期:2024-04-09 修回日期:2024-06-03 出版日期:2024-08-28 发布日期:2024-10-17
  • 通讯作者: Yi Xiao,E-mail:xiaoyi@dlut.edu.cn;Xinfu Zhang,E-mail:zhangxinfu@dlut.edu.cn
  • 基金资助:
    This work was supported by the National Natural Science Foundation of China (22278059, 22174009, and 22078047), Fundamental Research Funds for the Central Universities (DUT22LAB601 and DUT22LAB608).

Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Yucong Gong1, Xiangli Li1, Daqing Ma2, Lai Wang1, Lin Zhou1, Caiwei Lu1, Yi Xiao1, Xinfu Zhang1   

  1. 1 State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
    2 China Academy of Safety Science and Technology, Beijing 100012, China
  • Received:2024-04-09 Revised:2024-06-03 Online:2024-08-28 Published:2024-10-17
  • Contact: Yi Xiao,E-mail:xiaoyi@dlut.edu.cn;Xinfu Zhang,E-mail:zhangxinfu@dlut.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (22278059, 22174009, and 22078047), Fundamental Research Funds for the Central Universities (DUT22LAB601 and DUT22LAB608).

摘要: The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells. However, most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time. Herein, Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time. This tracker is composed of a lipophilic boron-dipyrromethene (BODIPY) derivative and a hydrophilic zwitterion to form an amphiphilic structure, which enables its targeting ability toward cell membranes. Moreover, a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically, which extends retention time in cell membranes. Mem580-F-Sulfo shows intense brightness (94600), with a high molar absorption coefficient of up to about 100000 L·mol-1·cm-1 and a fluorescence quantum yield of up to 0.97. It shows fast cell membrane targeting ability and long retention up to 90 min. In brief, this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

关键词: Plasma membrane, Protein, Tracker, Imaging, Microstructure

Abstract: The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells. However, most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time. Herein, Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time. This tracker is composed of a lipophilic boron-dipyrromethene (BODIPY) derivative and a hydrophilic zwitterion to form an amphiphilic structure, which enables its targeting ability toward cell membranes. Moreover, a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically, which extends retention time in cell membranes. Mem580-F-Sulfo shows intense brightness (94600), with a high molar absorption coefficient of up to about 100000 L·mol-1·cm-1 and a fluorescence quantum yield of up to 0.97. It shows fast cell membrane targeting ability and long retention up to 90 min. In brief, this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

Key words: Plasma membrane, Protein, Tracker, Imaging, Microstructure