SCI和EI收录∣中国化工学会会刊

›› 2014, Vol. 22 ›› Issue (11/12): 1328-1332.DOI: 10.1016/j.cjche.2014.09.026

• 生物技术与生物工程 • 上一篇    下一篇

Enzyme-catalyzed Sequential Reduction of Carbon Dioxide to Formaldehyde

Wenfang Liu, Yanhui Hou, Benxiang Hou, Zhiping Zhao   

  1. School of Chemical Engineering and the Environment, Beijing Institute of Technology, Beijing 100081, China
  • 收稿日期:2013-06-25 修回日期:2013-12-12 出版日期:2014-12-28 发布日期:2014-12-24
  • 通讯作者: Wenfang Liu
  • 基金资助:
    Supported by the National Natural Science Foundation of China (20806009, 20976012), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20070007055, 20091101110035).

Enzyme-catalyzed Sequential Reduction of Carbon Dioxide to Formaldehyde

Wenfang Liu, Yanhui Hou, Benxiang Hou, Zhiping Zhao   

  1. School of Chemical Engineering and the Environment, Beijing Institute of Technology, Beijing 100081, China
  • Received:2013-06-25 Revised:2013-12-12 Online:2014-12-28 Published:2014-12-24
  • Supported by:
    Supported by the National Natural Science Foundation of China (20806009, 20976012), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (20070007055, 20091101110035).

摘要: It has been reported that enzymatic-catalyzed reduction of CO2 is feasible.Most of literature focuses on the conversion of CO2 to methanol.Hereinwe put emphasis on the sequential conversion of CO2 to formaldehyde and its single reactions. It appears that CO2 pressure plays a critical role and higher pressure is greatly helpful to form more HCOOH as well as HCHO. The reverse reaction became severe in the reduction of CO2 to formaldehyde after 10 h, decreasing HCHO production. Increasing the mass ratio of formate dehydrogenase to formaldehyde dehydrogenase could promote the sequential reaction. At concentrations of nicotinamide adenine dinucleotide lower than 100 mmol·L-1, the reduction of CO2 was accelerated by increasing cofactor concentration. The optimum pH value and concentration of phosphate buffer were determined as 6.0 and 0.05 mol·L-1, respectively, for the overall reaction. It seems that thermodynamic factor such as pH is restrictive to the sequential reaction due to distinct divergence in appropriate pH range between its single reactions.

关键词: Carbon dioxide, Formic acid, Formaldehyde, Enzymatic-catalyzed, Sequential reduction

Abstract: It has been reported that enzymatic-catalyzed reduction of CO2 is feasible.Most of literature focuses on the conversion of CO2 to methanol.Hereinwe put emphasis on the sequential conversion of CO2 to formaldehyde and its single reactions. It appears that CO2 pressure plays a critical role and higher pressure is greatly helpful to form more HCOOH as well as HCHO. The reverse reaction became severe in the reduction of CO2 to formaldehyde after 10 h, decreasing HCHO production. Increasing the mass ratio of formate dehydrogenase to formaldehyde dehydrogenase could promote the sequential reaction. At concentrations of nicotinamide adenine dinucleotide lower than 100 mmol·L-1, the reduction of CO2 was accelerated by increasing cofactor concentration. The optimum pH value and concentration of phosphate buffer were determined as 6.0 and 0.05 mol·L-1, respectively, for the overall reaction. It seems that thermodynamic factor such as pH is restrictive to the sequential reaction due to distinct divergence in appropriate pH range between its single reactions.

Key words: Carbon dioxide, Formic acid, Formaldehyde, Enzymatic-catalyzed, Sequential reduction