Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

doi:10.1016/j.cjche.2016.08.027

›› 2017, Vol. 25 ›› Issue (4): 487-492.DOI: 10.1016/j.cjche.2016.08.027

• Biotechnology and Bioengineering • 上一篇下一篇

Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

Liya Zhou^1,2, Haixia Mou¹, Jing Gao^1,2, Li Ma¹, Ying He¹, Yanjun Jiang^1,2

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology, Tianjin 300130, China

收稿日期:2016-05-19 修回日期:2016-08-27 出版日期:2017-04-28 发布日期:2017-06-03
通讯作者: Liya Zhou, Jing Gao
基金资助:
Supported by the National Nature Science Foundation of China (Nos.21306039,21276060,21276062),the Natural Science Foundation of Hebei Province (B2015202082,B2016202027) and the Tianjin City High School Science&Technology Fund Planning Project (20140513).

Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

Liya Zhou^1,2, Haixia Mou¹, Jing Gao^1,2, Li Ma¹, Ying He¹, Yanjun Jiang^1,2

1 School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China;
2 Hebei Provincial Key Lab of Green Chemical Technology and High Efficient Energy Saving, Hebei University of Technology, Tianjin 300130, China

Received:2016-05-19 Revised:2016-08-27 Online:2017-04-28 Published:2017-06-03
Supported by:
Supported by the National Nature Science Foundation of China (Nos.21306039,21276060,21276062),the Natural Science Foundation of Hebei Province (B2015202082,B2016202027) and the Tianjin City High School Science&Technology Fund Planning Project (20140513).

摘要/Abstract

摘要： Cross-linked enzyme aggregates (CLEAs) of nitrile hydratase (NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time.In this process,egg white was added as protein feeder for facilitating the formation of CLEAs.The optimal conditions of the immobilization process were determined.Michaelis constants (K_m) of free NHase and NHase CLEAs were also determined.The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart.When exposed to high concentrations of acrylamide,NHase CLEAs also exhibited effective catalytic activity.

关键词: Immobilization, Enzyme, Biocatalysis, Nitrile hydratase

Abstract: Cross-linked enzyme aggregates (CLEAs) of nitrile hydratase (NHase) ES-NHT-118 from Escherichia coli were prepared by using ammonium sulfate as precipitating agent followed by cross-linking with dextran polyaldehyde for the first time.In this process,egg white was added as protein feeder for facilitating the formation of CLEAs.The optimal conditions of the immobilization process were determined.Michaelis constants (K_m) of free NHase and NHase CLEAs were also determined.The NHase CLEAs exhibited increased stability at varied pH and temperature conditions compared to its free counterpart.When exposed to high concentrations of acrylamide,NHase CLEAs also exhibited effective catalytic activity.

Key words: Immobilization, Enzyme, Biocatalysis, Nitrile hydratase

Liya Zhou, Haixia Mou, Jing Gao, Li Ma, Ying He, Yanjun Jiang. Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent[J]. , 2017, 25(4): 487-492.

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Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

Preparation of cross-linked enzyme aggregates of nitrile hydratase ES-NHT-118 from E. coli by macromolecular cross-linking agent

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