Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO2 foaming

doi:10.1016/j.cjche.2017.04.005

Chinese Journal of Chemical Engineering ›› 2018, Vol. 26 ›› Issue (1): 207-212.DOI: 10.1016/j.cjche.2017.04.005

• Materials and Product Engineering • 上一篇下一篇

Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

Xin Xin, Yixin Guan, Shanjing Yao

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:2017-02-10 修回日期:2017-04-07 出版日期:2018-01-28 发布日期:2018-03-01
通讯作者: Yixin Guan,E-mail address:guanyx@zju.edu.cn
基金资助:
Support by the National Natural Science Foundation of China (21276225, 21476196).

Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

Xin Xin, Yixin Guan, Shanjing Yao

College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China

Received:2017-02-10 Revised:2017-04-07 Online:2018-01-28 Published:2018-03-01
Contact: Yixin Guan,E-mail address:guanyx@zju.edu.cn
Supported by:
Support by the National Natural Science Foundation of China (21276225, 21476196).

摘要/Abstract

摘要： Scaffolds with multimodal pore structure are essential to cells differentiation and proliferation in bone tissue engineering. Bi-/multi-modal porous PLGA/hydroxyapatite composite scaffolds were prepared by supercritical CO₂ foaming in which hydroxyapatite acted as heterogeneous nucleation agent. Bimodal porous scaffolds were prepared under certain conditions, i.e. hydroxyapatite addition of 5%, depressurization rate of 0.3 MPa·min^-1, soaking temperature of 55℃, and pressure of 9 MPa. And scaffolds presented specific structure of small pores (122 μm±66 μm) in the cellular walls of large pores (552 μm±127 μm). Furthermore, multimodal porous PLGA scaffolds with micro-pores (37 μm±11 μm) were obtained at low soaking pressure of 7.5 MPa. The interconnected porosity of scaffolds ranged from (52.53±2.69)% to (83.08±2.42)% by adjusting depressurization rate, while compression modulus satisfied the requirement of bone tissue engineering. Solvent-free CO₂ foaming method is promising to fabricate bi-/multi-modal porous scaffolds in one step, and bioactive particles for osteogenesis could serve as nucleation agents.

关键词: Supercritical carbon dioxide, Foam, Tissue engineering, PLGA scaffolds, Hydroxyapatite, Bi-/multi-modal pore

Abstract: Scaffolds with multimodal pore structure are essential to cells differentiation and proliferation in bone tissue engineering. Bi-/multi-modal porous PLGA/hydroxyapatite composite scaffolds were prepared by supercritical CO₂ foaming in which hydroxyapatite acted as heterogeneous nucleation agent. Bimodal porous scaffolds were prepared under certain conditions, i.e. hydroxyapatite addition of 5%, depressurization rate of 0.3 MPa·min^-1, soaking temperature of 55℃, and pressure of 9 MPa. And scaffolds presented specific structure of small pores (122 μm±66 μm) in the cellular walls of large pores (552 μm±127 μm). Furthermore, multimodal porous PLGA scaffolds with micro-pores (37 μm±11 μm) were obtained at low soaking pressure of 7.5 MPa. The interconnected porosity of scaffolds ranged from (52.53±2.69)% to (83.08±2.42)% by adjusting depressurization rate, while compression modulus satisfied the requirement of bone tissue engineering. Solvent-free CO₂ foaming method is promising to fabricate bi-/multi-modal porous scaffolds in one step, and bioactive particles for osteogenesis could serve as nucleation agents.

Key words: Supercritical carbon dioxide, Foam, Tissue engineering, PLGA scaffolds, Hydroxyapatite, Bi-/multi-modal pore

Xin Xin, Yixin Guan, Shanjing Yao. Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming[J]. Chinese Journal of Chemical Engineering, 2018, 26(1): 207-212.

参考文献

[1] A.J. Salgado, O.P. Coutinho, R.L. Reis, Bone tissue engineering:State of the art and future trends, Macromol. Biosci. 4(2004) 743-765.

[2] R. Langer, J. Vacanti, Tissue engineering, Science 260(1993) 920-926.

[3] N. Harada, Y. Watanabe, K. Sato, S. Abe, K. Yamanaka, Y. Sakai, T. Kaneko, T. Matsushita, Bone regeneration in a massive rat femur defect through endochondral ossification achieved with chondrogenically differentiated MSCs in a degradable scaffold, Biomaterials 35(2014) 7800-7810.

[4] V. Karageorgiou, D. Kaplan, Porosity of 3D biomaterial scaffolds and osteogenesis, Biomaterials 26(2005) 5474-5491.

[5] A. Salerno, S. Zeppetelli, E. Di Maio, S. Iannace, P.A. Netti, Processing/structure/property relationship of multi-scaled PCL and PCL-HA composite scaffolds prepared via gas foaming and NaCl reverse templating, Biotechnol. Bioeng. 108(2011) 963-976.

[6] H.Y. Mi, X. Jing, L.S. Turng, Fabrication of porous synthetic polymer scaffolds for tissue engineering, J. Cell. Plast. 51(2014) 165-196.

[7] R.A. Quirk, R.M. France, K.M. Shakesheff, S.M. Howdle, Supercritical fluid technologies and tissue engineering scaffolds, Curr. Opin. Solid State Mater. Sci. 8(2004) 313-321.

[8] D.J. Mooney, D.F. Baldwin, N.P. Suh, L.P. Vacanti, R. Langer, Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents, Biomaterials 17(1996) 1417-1422.

[9] A. Salerno, S. Zeppetelli, E. Di Maio, S. Iannace, P.A. Netti, Architecture and properties of bi-modal porous scaffolds for bone regeneration prepared via supercritical CO₂ foaming and porogen leaching combined process, J. Supercrit. Fluids 67(2012) 114-122.

[10] L.D. Harris, B.S. Kim, D.J. Mooney, Open pore biodegradable matrices formed with gas foaming, J. Biomed. Mater. Res. 42(1998) 396-402.

[11] A. Salerno, S. Iannace, P.A. Netti, Graded biomimetic osteochondral scaffold prepared via CO₂ foaming and micronized NaCl leaching, Mater. Lett. 82(2012) 137-140.

[12] X. Xin, Q.Q. Liu, C.X. Chen, Y.X. Guan, S.J. Yao, Fabrication of bimodal porous PLGA scaffolds by supercritical CO₂ foaming/particle leaching technique, J. Appl. Polym. Sci. 33(2016) 43644.

[13] J. Colton, N. Suh, The nucleation of microcellular thermoplastic foam with additives. Part I:Theoretical considerations, Polym. Eng. Sci. 27(1987) 485-492.

[14] J. Colton, N. Suh, The nucleation of microcellular thermoplastic foam with additives. Part Ⅱ:Experimental results and discussion, Polym. Eng. Sci. 27(1987) 493-499.

[15] J. Colton, N. Suh, Nucleation of microcellular foam theory and practice, Polym. Eng. Sci. 27(1987) 500-503.

[16] I. Tsivintzelis, E. Pavlidou, C. Panayiotou, Biodegradable polymer foams prepared with supercritical CO₂-ethanol mixtures as blowing agents, J. Supercrit. Fluids 42(2007) 265-272.

[17] L.M. Mathieu, T.L. Mueller, P.E. Bourban, D.P. Pioletti, R. Muller, J.A. Manson, Architecture and properties of anisotropic polymer composite scaffolds for bone tissue engineering, Biomaterials 27(2006) 905-916.

[18] L. Mathieu, P. Bourban, J. Manson, Processing of homogeneous ceramic/polymer blends for bioresorbable composites, Compos. Sci. Technol. 66(2006) 1606-1614.

[19] M.Salarian,W.Z. Xu, Z.Wang,T.K.Sham, P.A. Charpentier, Hydroxyapatite-TiO₂-based nanocompositessynthesized in supercriticalCO₂forbonetissueengineering:Physical and mechanical properties, ACS Appl. Mater. Interfaces 6(2014) 16918-16931.

[20] A.M. Ng, K.K. Tan, M.Y. Phang, O. Aziyati, G.H. Tan, M.R. Isa, B.S. Aminuddin, M. Naseem, O. Fauziah, B.H. Ruszymah, Differential osteogenic activity of osteoprogenitor cells on HA and TCP/HA scaffold of tissue engineered bone, J. Biomed. Mater. Res. A 85(2008) 301-312.

[21] M. Bhamidipati, A.M. Scurto, M.S. Detamore, The future of carbon dioxide for polymer processing in tissue engineering, Tissue Eng. Part B Rev. 19(2013) 221-232.

[22] L.M. Mathieu, M.O. Montjovent, P.E. Bourban, D.P. Pioletti, J.A. Manson, Bioresorbable composites prepared by supercritical fluid foaming, J. Biomed. Mater. Res. A 75(2005) 89-97.

[23] G.P. Chen, T. Ushida, T. Tateishi, A biodegradable hybrid sponge nested with collagen microsponges, J. Biomed. Mater. Res. 51(2000) 273-279.

[24] N.S. Ramesh, Don H. Rasmussen, G.A. Campbell, The heterogeneous nucleation of microcellular foams assisted by the survival of microvoids in polymers containing low glass transition particles. Part I:Mathematical modeling and numerical simulation, Polym. Eng. Sci. 34(1994) 1685-1697.

[25] N.S. Ramesh, Don H. Rasmussen, G.A. Campbell, The heterogeneous nucleation of microcellular foams assisted by the survival of microvoids in polymers containing low glass transition particles. Part Ⅱ:Experimental results and discussion, Polym. Eng. Sci. 34(1994) 1698-1706.

[26] I. Tsivintzelis, A.G. Angelopoulou, C. Panayiotou, Foaming of polymers with supercritical CO₂:An experimental and theoretical study, Polymer 48(2007) 5928-5939.

[27] E. Reverchon, S. Cardea, Supercritical fluids in 3-D tissue engineering, J. Supercrit. Fluids 69(2012) 97-107.

[28] H.Y. Tai, M.L. Mather, D. Howard, W.X. Wang, L.J. White, J.A. Crowe, S.P. Morgan, A. Chandra, D.J. Williams, S.M. Howdle, K.M. Shakesheff, Control of pore size and structure of tissue engineering scaffolds produced by supercritical fluid processing, Eur. Cells Mater. 14(2007) 64-77.

[29] J.J. Barry, H.S. Gidda, C.A. Scotchford, S.M. Howdle, Porous methacrylate scaffolds:Supercritical fluid fabrication and in vitro chondrocyte responses, Biomaterials 25(2004) 3559-3568.

[30] C. Marrazzo, E. Di Maio, S. Iannace, Conventional and nanometric nucleating agents in poly(ε-caprolactone) foaming:Crystals vs. bubbles nucleation, Polym. Eng. Sci. 48(2008) 336-344.

[31] S.J. Hollister, Porous scaffold design for tissue engineering, Nat. Mater. 4(2005) 518-524.

编辑推荐 0

Metrics

阅读次数

全文

HTML			PDF

最新录用	在线预览	正式出版	最新录用	在线预览	正式出版
0	0	0	0	0	40

来源	本网站	其他网站

次数	21	19
比例	52%	48%

摘要

990

最新录用	在线预览	正式出版

0	0	990

来源	本网站	其他网站

次数	71	919
比例	7%	93%

Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

Bi-/multi-modal pore formation of PLGA/hydroxyapatite composite scaffolds by heterogeneous nucleation in supercritical CO₂ foaming

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐 0

Metrics

本文评价

[1]	Xiaolin Pan, Mengyuan Gao, Yun Wang, Yanping He, Tian Si, Yanlin Sun. Poly(lactic acid)-aspirin microspheres prepared via the traditional and improved solvent evaporation methods and its application performances[J]. 中国化学工程学报, 2023, 60(8): 194-204.
[2]	Shutong Pang, Hualiang An, Xinqiang Zhao, Yanji Wang. Influence of Ca/P ratio on the catalytic performance of hydroxyapatite for decarboxylation of itaconic acid to methacrylic acid[J]. 中国化学工程学报, 2023, 53(1): 402-408.
[3]	Xiaoyue Yao, Yu Liu, Zhenyu Chu, Wanqin Jin. Membranes for the life sciences and their future roles in medicine[J]. 中国化学工程学报, 2022, 49(9): 1-20.
[4]	Yue Wang, Luyao Huan, Haiyan Liang, Xuejia Ding, Jianguo Mi. Foaming biocompatible and biodegradable PBAT/PLGA as fallopian tube stent using supercritical carbon dioxide[J]. 中国化学工程学报, 2022, 47(7): 245-253.
[5]	Ye Zhang, Yong Gao, Peng Wang, Duo Na, Zhenming Yang, Jinsong Zhang. Solvent extraction with a three-dimensional reticulated hollow-strut SiC foam microchannel reactor[J]. 中国化学工程学报, 2022, 46(6): 53-62.
[6]	Yaling Li, Hao Ai, Liangzhi Qiao, Yinghong Wang, Kaifeng Du. Fabrication and characterization of hierarchical porous Ni²⁺ doped hydroxyapatite microspheres and their enhanced protein adsorption capacity[J]. 中国化学工程学报, 2022, 45(5): 238-247.
[7]	Wenbo Yang, Qingyun Li, Shiqi Guo, Shijie Sun, Aixing Tang, Haibo Liu, Youyan Liu. Rational design of Aspergillus flavus A5p1-immobilized cell system to enhance the decolorization of reactive blue 4 (RB4)[J]. 中国化学工程学报, 2022, 52(12): 37-44.
[8]	Anshi Hong, Zisheng Zhang, Xingang Li, Xin Gao. A generalized CFD model for evaluating catalytic separation process in structured porous materials[J]. 中国化学工程学报, 2022, 51(11): 168-177.
[9]	Zichao Hu, Chao Li, Dengfeng Zhang. Interactions of dynamic supercritical CO₂ fluid with different rank moisture-equilibrated coals: Implications for CO₂ sequestration in coal seams[J]. 中国化学工程学报, 2021, 35(7): 288-301.
[10]	Abbas Bambaeero, Reza Bazargan-Lari. Simultaneous removal of copper and zinc ions by low cost natural snail shell/hydroxyapatite/chitosan composite[J]. 中国化学工程学报, 2021, 33(5): 221-230.
[11]	Chi Ma, Le Sang, Xiaonan Duan, Jiabin Yin, Jisong Zhang. An efficient method for enhancing adhesion and uniformity of Al₂O₃ coatings on nickel micro-foam used in micropacked beds[J]. 中国化学工程学报, 2021, 39(11): 162-172.
[12]	Yang Yu, Guiying Li, Wanqing Han, Linhua Zhu, Tian Si, Hong Wang, Yanlin Sun, Yanping He. An efficient preparation of porous polymeric microspheres by solvent evaporation in foam phase[J]. 中国化学工程学报, 2021, 29(1): 409-416.
[13]	Chaobo Song, Jiapeng Zhang, Shuang Li, Shengbin Yang, Eryi Lu, Zhenhao Xi, Lian Cen, Ling Zhao, Weikang Yuan. Highly interconnected macroporous MBG/PLGA scaffolds with enhanced mechanical and biological properties via green foaming strategy[J]. 中国化学工程学报, 2021, 29(1): 426-436.
[14]	Yanan Guan, Hengyu Shen, Xing Guo, Boyang Mao, Zhenyuan Yang, Yangtao Zhou, He Liang, Xiaolei Fan, Yilai Jiao, Jinsong Zhang. Structured hierarchical Mn-Co mixed oxides supported on silicalite-1 foam catalyst for catalytic combustion[J]. 中国化学工程学报, 2020, 28(9): 2319-2327.
[15]	Rui Zheng, Chunhu Li, Chengzhen Zhang, Wentai Wang, Liang Wang, Lijuan Feng, Junjie Bian. Photo-reduction of NO by g-C₃N₄@foamed ceramic[J]. 中国化学工程学报, 2020, 28(7): 1840-1846.