SCI和EI收录∣中国化工学会会刊

Chinese Journal of Chemical Engineering ›› 2019, Vol. 27 ›› Issue (2): 411-417.DOI: 10.1016/j.cjche.2018.03.034

• Biotechnology and Bioengineering • 上一篇    下一篇

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

Xiaoqiang Jia1,2,3, Yun He1, Lei Huang1, Dawei Jiang1, Wenyu Lu1,2,3   

  1. 1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
    2 Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
    3 Synthetic Biology Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China
  • 收稿日期:2018-01-11 修回日期:2018-03-23 出版日期:2019-02-28 发布日期:2019-03-18
  • 通讯作者: Wenyu Lu
  • 基金资助:

    Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), and Tianjin Key Research & Development Program (16YFXTSF00460)

n-Hexadecane and pyrene biodegradation and metabolization by Rhodococcus sp. T1 isolated from oil contaminated soil

Xiaoqiang Jia1,2,3, Yun He1, Lei Huang1, Dawei Jiang1, Wenyu Lu1,2,3   

  1. 1 Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China;
    2 Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin 300072, China;
    3 Synthetic Biology Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300072, China
  • Received:2018-01-11 Revised:2018-03-23 Online:2019-02-28 Published:2019-03-18
  • Contact: Wenyu Lu
  • Supported by:

    Supported by the National Basic Research Program of China (“973” Program: 2014CB745100), the National Natural Science Foundation of China (21576197), and Tianjin Key Research & Development Program (16YFXTSF00460)

摘要: The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n-hexadecane are both difficult to degrade. In this study, n-hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n-hexadecane (2 vol%) and 42.79% pyrene (200 mg·L-1) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n-hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35℃, 0.5% NaCl, 5 mg·L-1 of yeast extract and 90 mg·L-1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.

关键词: Biodegradation, Metabolite, n-Hexadecane, Pyrene, Rhodococcus sp.T1

Abstract: The high-molecular weight polycyclic aromatic hydrocarbons (PAHs) pyrene and typical long chain alkane n-hexadecane are both difficult to degrade. In this study, n-hexadecane and pyrene degrading strain Rhodococcus sp. T1 was isolated from oil contaminated soil. Strain T1 could remove 90.81% n-hexadecane (2 vol%) and 42.79% pyrene (200 mg·L-1) as a single carbon within 5 days, respectively. Comparatively, the degradation of pyrene increased to 60.63%, but the degradation of n-hexadecane decreased to 87.55% when these compounds were mixed. Additionally, identification and analysis of degradation metabolites of Rhodococcus sp. T1 in the above experiments showed that there were significant changes in alanine, methylamine, citric acid and heptadecanoic acid between sole and dual substrate degradation. The optimal conditions for degradation were then determined based on analysis of the pH, salinity, additional nutrient sources and liquid surface activity. Under the optimal conditions of pH 7.0, 35℃, 0.5% NaCl, 5 mg·L-1 of yeast extract and 90 mg·L-1 of surfactant, the degradation increased in single or dual carbon sources. To our knowledge, this is the first study to discuss metabolite changes in Rhodococcus sp. T1 using sole substrate and dual substrate to enhance the long-chain alkanes and PAHs degradation potential.

Key words: Biodegradation, Metabolite, n-Hexadecane, Pyrene, Rhodococcus sp.T1