Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

doi:10.1016/j.cjche.2020.08.014

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (12): 3103-3109.DOI: 10.1016/j.cjche.2020.08.014

• Biotechnology and Bioengineering • 上一篇下一篇

Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

Mengmeng Wang¹, Gang Cao¹, Naixian Feng², Yongzhang Pan¹

1 College of Environment, Jinan University, Guangzhou 511443, China;
2 Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

收稿日期:2019-12-02 修回日期:2020-08-06 出版日期:2020-12-28 发布日期:2021-01-11
通讯作者: Gang Cao
基金资助:
This work was supported by grants from the Science and Technology Planning Project of Guangzhou City, China (201903010031) and the Natural Science Foundation Research Team Project of Guangdong Province, China (2016A030312009).

Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

Mengmeng Wang¹, Gang Cao¹, Naixian Feng², Yongzhang Pan¹

1 College of Environment, Jinan University, Guangzhou 511443, China;
2 Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China

Received:2019-12-02 Revised:2020-08-06 Online:2020-12-28 Published:2021-01-11
Contact: Gang Cao
Supported by:
This work was supported by grants from the Science and Technology Planning Project of Guangzhou City, China (201903010031) and the Natural Science Foundation Research Team Project of Guangdong Province, China (2016A030312009).

摘要/Abstract

摘要： Mixed strains Delftia sp.YH01 and Acidovorax sp.YH02, with capability of heterotrophic nitrification-aerobic denitrification, were introduced into a two-stage aerobic sequencing batch reactor to enhance NO₃^--N removal. With optimal C/N of 8, efficient NO₃^--N removal was achieved at initial NO₃^--N concentration of 2000 mg·L^-1. Meanwhile, the massive accumulation of NO₂^--N was avoided during the long operation. Compared to the one-stage aerobic sequencing batch reactor, the removal efficiency of NO₃^--N and TN in the two-stage aerobic sequencing batch reactor was increased by 36.5% and 42.7%, which respectively was 93.8% and 88.4%. Microbial community study showed that the mixed strains have the stronger viability and can synergistically denitrify with the indigenous microorganisms in system, such as Azoarcus, Uncultured Saprospiraceae, Thauera, Paracocccus, which could be major contributors for aerobic denitrification. The proposed technology was shown to achieve high-efficiency treatment of high NO₃^--N wastewater through aerobic denitrification.

关键词: Waste water, Biological engineering, Bioreactors, Aerobic denitrification, Bioaugmentation, Microbial community

Abstract: Mixed strains Delftia sp.YH01 and Acidovorax sp.YH02, with capability of heterotrophic nitrification-aerobic denitrification, were introduced into a two-stage aerobic sequencing batch reactor to enhance NO₃^--N removal. With optimal C/N of 8, efficient NO₃^--N removal was achieved at initial NO₃^--N concentration of 2000 mg·L^-1. Meanwhile, the massive accumulation of NO₂^--N was avoided during the long operation. Compared to the one-stage aerobic sequencing batch reactor, the removal efficiency of NO₃^--N and TN in the two-stage aerobic sequencing batch reactor was increased by 36.5% and 42.7%, which respectively was 93.8% and 88.4%. Microbial community study showed that the mixed strains have the stronger viability and can synergistically denitrify with the indigenous microorganisms in system, such as Azoarcus, Uncultured Saprospiraceae, Thauera, Paracocccus, which could be major contributors for aerobic denitrification. The proposed technology was shown to achieve high-efficiency treatment of high NO₃^--N wastewater through aerobic denitrification.

Key words: Waste water, Biological engineering, Bioreactors, Aerobic denitrification, Bioaugmentation, Microbial community

Mengmeng Wang, Gang Cao, Naixian Feng, Yongzhang Pan. Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment[J]. 中国化学工程学报, 2020, 28(12): 3103-3109.

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Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

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