SCI和EI收录∣中国化工学会会刊

中国化学工程学报 ›› 2020, Vol. 28 ›› Issue (12): 3103-3109.DOI: 10.1016/j.cjche.2020.08.014

• Biotechnology and Bioengineering • 上一篇    下一篇

Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

Mengmeng Wang1, Gang Cao1, Naixian Feng2, Yongzhang Pan1   

  1. 1 College of Environment, Jinan University, Guangzhou 511443, China;
    2 Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
  • 收稿日期:2019-12-02 修回日期:2020-08-06 出版日期:2020-12-28 发布日期:2021-01-11
  • 通讯作者: Gang Cao
  • 基金资助:
    This work was supported by grants from the Science and Technology Planning Project of Guangzhou City, China (201903010031) and the Natural Science Foundation Research Team Project of Guangdong Province, China (2016A030312009).

Bioaugmentation of two-stage aerobic sequencing batch reactor with mixed strains for high nitrate nitrogen wastewater treatment

Mengmeng Wang1, Gang Cao1, Naixian Feng2, Yongzhang Pan1   

  1. 1 College of Environment, Jinan University, Guangzhou 511443, China;
    2 Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and Technology, Jinan University, Guangzhou 510632, China
  • Received:2019-12-02 Revised:2020-08-06 Online:2020-12-28 Published:2021-01-11
  • Contact: Gang Cao
  • Supported by:
    This work was supported by grants from the Science and Technology Planning Project of Guangzhou City, China (201903010031) and the Natural Science Foundation Research Team Project of Guangdong Province, China (2016A030312009).

摘要: Mixed strains Delftia sp.YH01 and Acidovorax sp.YH02, with capability of heterotrophic nitrification-aerobic denitrification, were introduced into a two-stage aerobic sequencing batch reactor to enhance NO3--N removal. With optimal C/N of 8, efficient NO3--N removal was achieved at initial NO3--N concentration of 2000 mg·L-1. Meanwhile, the massive accumulation of NO2--N was avoided during the long operation. Compared to the one-stage aerobic sequencing batch reactor, the removal efficiency of NO3--N and TN in the two-stage aerobic sequencing batch reactor was increased by 36.5% and 42.7%, which respectively was 93.8% and 88.4%. Microbial community study showed that the mixed strains have the stronger viability and can synergistically denitrify with the indigenous microorganisms in system, such as Azoarcus, Uncultured Saprospiraceae, Thauera, Paracocccus, which could be major contributors for aerobic denitrification. The proposed technology was shown to achieve high-efficiency treatment of high NO3--N wastewater through aerobic denitrification.

关键词: Waste water, Biological engineering, Bioreactors, Aerobic denitrification, Bioaugmentation, Microbial community

Abstract: Mixed strains Delftia sp.YH01 and Acidovorax sp.YH02, with capability of heterotrophic nitrification-aerobic denitrification, were introduced into a two-stage aerobic sequencing batch reactor to enhance NO3--N removal. With optimal C/N of 8, efficient NO3--N removal was achieved at initial NO3--N concentration of 2000 mg·L-1. Meanwhile, the massive accumulation of NO2--N was avoided during the long operation. Compared to the one-stage aerobic sequencing batch reactor, the removal efficiency of NO3--N and TN in the two-stage aerobic sequencing batch reactor was increased by 36.5% and 42.7%, which respectively was 93.8% and 88.4%. Microbial community study showed that the mixed strains have the stronger viability and can synergistically denitrify with the indigenous microorganisms in system, such as Azoarcus, Uncultured Saprospiraceae, Thauera, Paracocccus, which could be major contributors for aerobic denitrification. The proposed technology was shown to achieve high-efficiency treatment of high NO3--N wastewater through aerobic denitrification.

Key words: Waste water, Biological engineering, Bioreactors, Aerobic denitrification, Bioaugmentation, Microbial community