Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

doi:10.1016/j.cjche.2024.06.002

中国化学工程学报 ›› 2024, Vol. 72 ›› Issue (8): 220-225.DOI: 10.1016/j.cjche.2024.06.002

Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Yucong Gong¹, Xiangli Li¹, Daqing Ma², Lai Wang¹, Lin Zhou¹, Caiwei Lu¹, Yi Xiao¹, Xinfu Zhang¹

1 State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
2 China Academy of Safety Science and Technology, Beijing 100012, China

收稿日期:2024-04-09 修回日期:2024-06-03 出版日期:2024-08-28 发布日期:2024-10-17
通讯作者: Yi Xiao,E-mail:xiaoyi@dlut.edu.cn;Xinfu Zhang,E-mail:zhangxinfu@dlut.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (22278059, 22174009, and 22078047), Fundamental Research Funds for the Central Universities (DUT22LAB601 and DUT22LAB608).

Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Yucong Gong¹, Xiangli Li¹, Daqing Ma², Lai Wang¹, Lin Zhou¹, Caiwei Lu¹, Yi Xiao¹, Xinfu Zhang¹

1 State Key Laboratory of Fine Chemicals, Frontiers Science Center for Smart Materials Oriented Chemical Engineering, Dalian University of Technology, Dalian 116024, China;
2 China Academy of Safety Science and Technology, Beijing 100012, China

Received:2024-04-09 Revised:2024-06-03 Online:2024-08-28 Published:2024-10-17
Contact: Yi Xiao,E-mail:xiaoyi@dlut.edu.cn;Xinfu Zhang,E-mail:zhangxinfu@dlut.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (22278059, 22174009, and 22078047), Fundamental Research Funds for the Central Universities (DUT22LAB601 and DUT22LAB608).

摘要/Abstract

摘要： The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells. However, most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time. Herein, Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time. This tracker is composed of a lipophilic boron-dipyrromethene (BODIPY) derivative and a hydrophilic zwitterion to form an amphiphilic structure, which enables its targeting ability toward cell membranes. Moreover, a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically, which extends retention time in cell membranes. Mem580-F-Sulfo shows intense brightness (94600), with a high molar absorption coefficient of up to about 100000 L·mol^-1·cm^-1 and a fluorescence quantum yield of up to 0.97. It shows fast cell membrane targeting ability and long retention up to 90 min. In brief, this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

关键词: Plasma membrane, Protein, Tracker, Imaging, Microstructure

Abstract: The construction of a stable-membrane tracker has significant implications for the visualization of the membrane in live cells. However, most current plasma trackers are not suitable for tracking plasma membranes for a long time due to their limited retention time. Herein, Mem580-F-Sulfo is designed to target and anchor cell membranes and therefore track cell membranes for a longer time. This tracker is composed of a lipophilic boron-dipyrromethene (BODIPY) derivative and a hydrophilic zwitterion to form an amphiphilic structure, which enables its targeting ability toward cell membranes. Moreover, a reactive ester group is included to bind with proteins through covalent bonds in cell membranes non-specifically, which extends retention time in cell membranes. Mem580-F-Sulfo shows intense brightness (94600), with a high molar absorption coefficient of up to about 100000 L·mol^-1·cm^-1 and a fluorescence quantum yield of up to 0.97. It shows fast cell membrane targeting ability and long retention up to 90 min. In brief, this work has not only developed a tracker with good cell membrane targetability but also provided a new strategy for improving the targeting stability of cell membranes.

Key words: Plasma membrane, Protein, Tracker, Imaging, Microstructure

Yucong Gong, Xiangli Li, Daqing Ma, Lai Wang, Lin Zhou, Caiwei Lu, Yi Xiao, Xinfu Zhang. Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene[J]. 中国化学工程学报, 2024, 72(8): 220-225.

Yucong Gong, Xiangli Li, Daqing Ma, Lai Wang, Lin Zhou, Caiwei Lu, Yi Xiao, Xinfu Zhang. Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene[J]. Chinese Journal of Chemical Engineering, 2024, 72(8): 220-225.

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Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

Plasma membrane-anchored fluorescent tracker based on boron-dipyrromethene

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