Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

›› 2011, Vol. 19 ›› Issue (4): 682-686.

Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

戴建英, 张媛丽, 修志龙

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

收稿日期:2011-01-18 修回日期:2011-04-04 出版日期:2011-08-28 发布日期:2011-08-28
通讯作者: XIU Zhilong,E-mail:zhlxiu@dlut.edu.cn
基金资助:
Supported by the National High Technology Research and Development Program of China (2009AA05Z443)

Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

DAI Jianying, ZHANG Yuanli, XIU Zhilong

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

Received:2011-01-18 Revised:2011-04-04 Online:2011-08-28 Published:2011-08-28
Supported by:
Supported by the National High Technology Research and Development Program of China (2009AA05Z443)

摘要/Abstract

摘要： The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol, an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study, we investigated the use of salting-out extraction(SOE) that employed a K₂HPO₄/ethanol system consisting of 21% ethanol and 17% K₂HPO₄(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE, about 98% of solid matters was removed, and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%, respectively, and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.

关键词: 2,3-butanediol, Jerusalem artichoke, salting-out extraction, viscosity

Abstract: The removal of solid impurities and separation of target products from a fermentation broth is becoming more tedious with the utilization of lignocelluloses as source of substrate.2,3-Butanediol, an important chemical used widely is also a main product of sugar-based fermentation carried out by Klebsiella pneumoniae.In this study, we investigated the use of salting-out extraction(SOE) that employed a K₂HPO₄/ethanol system consisting of 21% ethanol and 17% K₂HPO₄(mass fraction) to separate 2,3-butanediol from the viscous Jerusalem artichoke-based fermentation broth.After SOE, about 98% of solid matters was removed, and the viscosity decreased from 72.5 mPa s in the original fermentation broth to 4.4 mPa s in the top phase.The partition coefficient and yield of 2,3-butanediol reached 13.4 and 99%, respectively, and 89% of soluble proteins was removed from the broth.The results showed that SOE is an efficient way for isolating 2,3-BD from a highly viscous fermentation broth by removing much of the solid matters within the broth.

Key words: 2,3-butanediol, Jerusalem artichoke, salting-out extraction, viscosity

戴建英, 张媛丽, 修志龙. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth [J]. , 2011, 19(4): 682-686.

DAI Jianying, ZHANG Yuanli, XIU Zhilong. Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth [J]. , 2011, 19(4): 682-686.

参考文献

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Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

Salting-out Extraction of 2,3-Butanediol from Jerusalem artichoke-based Fermentation Broth

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