SCI和EI收录∣中国化工学会会刊

›› 2014, Vol. 22 ›› Issue (11/12): 1333-1339.DOI: 10.1016/j.cjche.2014.09.029

• 生物技术与生物工程 • 上一篇    下一篇

Lipase Immobilization onto the Surface of PGMA-b-PDMAEMA-grafted Magnetic Nanoparticles Prepared via Atom Transfer Radical Polymerization

Jingyun Wang1, Fangling Ji1, Jishuang Xing1, Shuang Cui1, Yongming Bao1, Wenbo Hao 2   

  1. 1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;
    2 Department of Physics and Chemistry, Heihe University, Heihe 164300, China
  • 收稿日期:2013-07-09 修回日期:2013-12-16 出版日期:2014-12-28 发布日期:2014-12-24
  • 通讯作者: Yongming Bao
  • 基金资助:
    Supported by the National Basic Research Program of China (2009CB724706).

Lipase Immobilization onto the Surface of PGMA-b-PDMAEMA-grafted Magnetic Nanoparticles Prepared via Atom Transfer Radical Polymerization

Jingyun Wang1, Fangling Ji1, Jishuang Xing1, Shuang Cui1, Yongming Bao1, Wenbo Hao 2   

  1. 1 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;
    2 Department of Physics and Chemistry, Heihe University, Heihe 164300, China
  • Received:2013-07-09 Revised:2013-12-16 Online:2014-12-28 Published:2014-12-24
  • Supported by:
    Supported by the National Basic Research Program of China (2009CB724706).

摘要: A block copolymer of 2-dimethylaminoethyl methacrylate (DMAEMA) and glycidyl methacrylate (GMA) was grafted onto the surface of magnetic nanoparticles (Fe3O4) via atom transfer radical polymerization. The resultant PGMA-b-PDMAEMA-grafted-Fe3O4 magnetic nanoparticles with amino and epoxy groups were characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, thermo-gravimetric analysis, and scanning electron microscopy. Lipase from Burkholderia cepacia was successfully immobilized onto the magnetic nanoparticles by physical adsorption and covalent bonding. The immobilization capacity of the magnetic particles is 0.5 mg lipase per mg support, with an activity recovery of up to 43.1% under the optimum immobilization condition. Biochemical characterization shows that the immobilized lipase exhibits improved thermal stability, good tolerance to organic solvents with high lg P, and higher pH stability than the free lipase at pH 9.0. After six consecutive cycles, the residual activity of the immobilized lipase is still over 55% of its initial activity.

关键词: Enzyme, Atom transfer radical polymerization, Immobilized lipase, Fe3O4 nanoparticles

Abstract: A block copolymer of 2-dimethylaminoethyl methacrylate (DMAEMA) and glycidyl methacrylate (GMA) was grafted onto the surface of magnetic nanoparticles (Fe3O4) via atom transfer radical polymerization. The resultant PGMA-b-PDMAEMA-grafted-Fe3O4 magnetic nanoparticles with amino and epoxy groups were characterized by Fourier transform infrared spectroscopy, powder X-ray diffraction, thermo-gravimetric analysis, and scanning electron microscopy. Lipase from Burkholderia cepacia was successfully immobilized onto the magnetic nanoparticles by physical adsorption and covalent bonding. The immobilization capacity of the magnetic particles is 0.5 mg lipase per mg support, with an activity recovery of up to 43.1% under the optimum immobilization condition. Biochemical characterization shows that the immobilized lipase exhibits improved thermal stability, good tolerance to organic solvents with high lg P, and higher pH stability than the free lipase at pH 9.0. After six consecutive cycles, the residual activity of the immobilized lipase is still over 55% of its initial activity.

Key words: Enzyme, Atom transfer radical polymerization, Immobilized lipase, Fe3O4 nanoparticles