Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

›› 2009, Vol. 17 ›› Issue (3): 500-505.

Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

肖诚斌¹, 宁君¹, 闫海², 孙旭东², 胡继业²

1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
2. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China

收稿日期:2008-10-06 修回日期:2009-02-28 出版日期:2009-06-28 发布日期:2009-06-28
通讯作者: YAN Hai,E-mail:haiyan@sas.ustb.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20777008);the Education Committee of Beijing

Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

XIAO Chengbin¹, NING Jun¹, YAN Hai², SUN Xudong², HU Jiye²

1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
2. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China

Received:2008-10-06 Revised:2009-02-28 Online:2009-06-28 Published:2009-06-28
Supported by:
Supported by the National Natural Science Foundation of China (20777008);the Education Committee of Beijing

摘要/Abstract

摘要： A promising gram-negative bacterial strain for the biodegradation of aniline as the sole carbon, nitrogen and energy sources was successfully isolated and identified as Delftia sp. XYJ6. The optimal temperature and pH for both the growth of Delftia sp. XYJ6 and the biodegradation of aniline were 30℃ and 7.0, respectively. Initial aniline of 2000 mg·L^-1 could be completely removed by the strain at 22 h, which showed that Delftia sp. XYJ6 had a strong ability in the biodegradation of aniline. It indicated that aniline was firstly converted to catechol catalyzed by aniline dioxygenase as a first product, which was then further biodegraded to cis,cis-muconic acid catalyzed by the catechol 1,2-dioxygenase of Delftia sp. XYJ6 as a second product. Cis,cis-muconic acid could also be further biodegraded to other small compound again. The pathway for the biodegradation of aniline by Delftia sp. XYJ6 was not previously reported.

关键词: aniline, Delftia sp., biodegradation, pathway

Abstract: A promising gram-negative bacterial strain for the biodegradation of aniline as the sole carbon, nitrogen and energy sources was successfully isolated and identified as Delftia sp. XYJ6. The optimal temperature and pH for both the growth of Delftia sp. XYJ6 and the biodegradation of aniline were 30℃ and 7.0, respectively. Initial aniline of 2000 mg·L^-1 could be completely removed by the strain at 22 h, which showed that Delftia sp. XYJ6 had a strong ability in the biodegradation of aniline. It indicated that aniline was firstly converted to catechol catalyzed by aniline dioxygenase as a first product, which was then further biodegraded to cis,cis-muconic acid catalyzed by the catechol 1,2-dioxygenase of Delftia sp. XYJ6 as a second product. Cis,cis-muconic acid could also be further biodegraded to other small compound again. The pathway for the biodegradation of aniline by Delftia sp. XYJ6 was not previously reported.

Key words: aniline, Delftia sp., biodegradation, pathway

肖诚斌, 宁君, 闫海, 孙旭东, 胡继业. Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6[J]. , 2009, 17(3): 500-505.

XIAO Chengbin, NING Jun, YAN Hai, SUN Xudong, HU Jiye. Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6[J]. , 2009, 17(3): 500-505.

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Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

Biodegradation of Aniline by a Newly Isolated Delftia sp. XYJ6

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