Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

›› 2011, Vol. 19 ›› Issue (5): 773-778.

Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

张名佳, 苏荣欣, 齐崴, 杜若愚, 何志敏

State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engi-neering and Technology, Tianjin University, Tianjin 300072, China

收稿日期:2011-06-10 修回日期:2011-07-13 出版日期:2011-10-28 发布日期:2011-10-28
通讯作者: HE Zhimin,E-mail:enzyme@tju.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20976130 and 20806057);National Science and Technology Pillar Program of China (2007BAD42B02);Program for New Century Excellent Talents in University of Ministry of Education of China (No.NCET-08-0386);the R&D program of Tianjin Binhai New Area (2010-BK17C004)

Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

ZHANG Mingjia, SU Rongxin, QI Wei, DU Ruoyu, HE Zhimin

State Key Laboratory of Chemical Engineering, Chemical Engineering Research Center, School of Chemical Engi-neering and Technology, Tianjin University, Tianjin 300072, China

Received:2011-06-10 Revised:2011-07-13 Online:2011-10-28 Published:2011-10-28
Supported by:
Supported by the National Natural Science Foundation of China (20976130 and 20806057);National Science and Technology Pillar Program of China (2007BAD42B02);Program for New Century Excellent Talents in University of Ministry of Education of China (No.NCET-08-0386);the R&D program of Tianjin Binhai New Area (2010-BK17C004)

摘要/Abstract

摘要： The reactions of exo-cellulase (cellobiohydrolase, CBH) and endo-cellulase (endoglucanase, EG) were investigated by analyzing the insoluble residues of microcrystalline cellulose (MCC) and filter paper cellulose (FPC) during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution, degree of po-lymerization, and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period, indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand, the degree of polymerization of FPC chains decreases rapidly in the initial reaction, indicating that EG digestion follows a random scission manner, which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually, indicating stronger chain stiffness of cellulose during hydrolysis.

关键词: cellulose, cellobiohydrolase, endoglucanase, relative molecular mass distribution, SEC-MALLS-DRI

Abstract: The reactions of exo-cellulase (cellobiohydrolase, CBH) and endo-cellulase (endoglucanase, EG) were investigated by analyzing the insoluble residues of microcrystalline cellulose (MCC) and filter paper cellulose (FPC) during enzymatic hydrolysis. Molecular parameters including molecular weight and its distribution, degree of po-lymerization, and radii of gyration were measured by size exclusion chromatography coupled with multi-angle laser light scattering. No significant change in MCC chains was found during the whole reaction period, indicating that CBH digestion follows a layer-by-layer solubilization manner. This reaction mode might be the major reason for slow enzymatic hydrolysis of cellulose. On the other hand, the degree of polymerization of FPC chains decreases rapidly in the initial reaction, indicating that EG digestion follows a random scission manner, which may create new ends for CBH easily. The slopes of the conformation plots for MCC and FPC increase gradually, indicating stronger chain stiffness of cellulose during hydrolysis.

Key words: cellulose, cellobiohydrolase, endoglucanase, relative molecular mass distribution, SEC-MALLS-DRI

张名佳, 苏荣欣, 齐崴, 杜若愚, 何志敏. Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS[J]. , 2011, 19(5): 773-778.

ZHANG Mingjia, SU Rongxin, QI Wei, DU Ruoyu, HE Zhimin. Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS[J]. , 2011, 19(5): 773-778.

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Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

Enzymatic Hydrolysis of Cellulose with Different Crystallinities Studied by Means of SEC-MALLS

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