Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2

›› 2008, Vol. 16 ›› Issue (5): 796-800.

Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2

姜岩^1,2,3, 任南琪², 蔡徇¹, 吴迪¹, 乔丽艳¹, 林森¹

1. Department of Chemical Engineering, Daqing Oilfield Engineering Limited Company, Daqing 163712, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;
3. School of Life Sciences and Chemistry, Harbin 150016, China

收稿日期:2008-01-24 修回日期:2008-05-15 出版日期:2008-10-28 发布日期:2008-10-28
通讯作者: JIANG Yan,E-mail:yjiang@hit.edu.cn
基金资助:
the Science and Technology Innovative Talents Foundation of China(2006RFQXS070);the Youth Academic Cadreman Project of Heilongjiang Province(1152G068);Scientific Research Fund of Heilongjiang Province(11523063);the Science Foundation for Post Doctorate of China(20070410268).

Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2

JIANG Yan^1,2,3, REN Nanqi², CAI Xun¹, WU Di¹, QIAO Liyan¹, LIN Sen¹

1. Department of Chemical Engineering, Daqing Oilfield Engineering Limited Company, Daqing 163712, China;
2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China;
3. School of Life Sciences and Chemistry, Harbin 150016, China

Received:2008-01-24 Revised:2008-05-15 Online:2008-10-28 Published:2008-10-28
Supported by:
the Science and Technology Innovative Talents Foundation of China(2006RFQXS070);the Youth Academic Cadreman Project of Heilongjiang Province(1152G068);Scientific Research Fund of Heilongjiang Province(11523063);the Science Foundation for Post Doctorate of China(20070410268).

摘要/Abstract

摘要： The biodegradations of phenol and 4-chlorophenol(4-cp)were studied using the mutant strain CTM 2 obtained by the He-Ne laser irradiation on wild-type Candidatropicalis.The results showed that the capacity of the CTM 2 to biodegrade 4-cp was increased up to 400 mg·L^-1 within 59.5 h.In the dual-substrate biodegradation,both velocity and capacity of the CTM 2 to degrade 4-cp increased with low-concentration phenol.A total of 400 mg·L^-1 4-cp was completely degraded within 50.5 h in the presence of 300 mg·L^-1 phenol.The maximum 4-cp biodegradation could reach 440 mg·L^-1 with 120 mg·L^-1 phenol.Low-concentration 4-cp caused great inhibition on the CTM 2 to degrade phenol.In addition,the kinetic behaviors were described using the kinetic model proposed in this lab.

关键词: biodegradation, phenol, 4-chlorophenol, the mutant strain CTM 2

Abstract: The biodegradations of phenol and 4-chlorophenol(4-cp)were studied using the mutant strain CTM 2 obtained by the He-Ne laser irradiation on wild-type Candidatropicalis.The results showed that the capacity of the CTM 2 to biodegrade 4-cp was increased up to 400 mg·L^-1 within 59.5 h.In the dual-substrate biodegradation,both velocity and capacity of the CTM 2 to degrade 4-cp increased with low-concentration phenol.A total of 400 mg·L^-1 4-cp was completely degraded within 50.5 h in the presence of 300 mg·L^-1 phenol.The maximum 4-cp biodegradation could reach 440 mg·L^-1 with 120 mg·L^-1 phenol.Low-concentration 4-cp caused great inhibition on the CTM 2 to degrade phenol.In addition,the kinetic behaviors were described using the kinetic model proposed in this lab.

Key words: biodegradation, phenol, 4-chlorophenol, the mutant strain CTM 2

姜岩, 任南琪, 蔡徇, 吴迪, 乔丽艳, 林森. Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2[J]. , 2008, 16(5): 796-800.

JIANG Yan, REN Nanqi, CAI Xun, WU Di, QIAO Liyan, LIN Sen. Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2[J]. , 2008, 16(5): 796-800.

参考文献

1 Kavitha, V., Palanivelu, K., “The role of ferrous ion in fenton and photo-fenton processes for the degradation of phenol”, Chemosphere, 55, 1235-1243 (2004).
2 Ji, X.L., Zhang, J.L., Li, W., Han, Z.T., Wang, Y.P., “Effect of substrate permeation on kinetics of phenol biodegradation”, Chin. J. Chem. Eng., 11 (2), 151-155 (2003).
3 Ai, Z., Yang, P., Lu, X.H., “Degradation of 4-chlorophenol by a microwave assisted photocatalysis method”, J. Hazard. Mater., 124, 147-152 (2005).
4 Zhao, D., Xu, X., Lei, L., Wang, D., “Degradation of 4-chlorophenol solution by synergetic effect of dual-frequency ultrasound with Fenton reagent”, Chin. J. Chem. Eng., 13 (2), 204-210 (2005).
5 Kim, J.H., Oh, K.K., Lee, S.T., “Biodegradation of phenol and chlorophenols with defined mixed culture in shake-flasks and a packed bed reactor”, Process Biochem., 37, 1367-1373 (2002).
6 Xu, X., Wang, D., “Catalytic wet air oxidation of o-chlorophenol in wastewater”, Chin. J. Chem. Eng., 11 (3), 352-354 (2003).
7 Yuan, S.H., Lu, X.H., “Comparison treatment of various chlorophenols by electro-Fenton method:Relationship between chlorine content and degradation”, J. Hazard. Mater., 118, 85-92 (2005).
8 Ye, F.X., Shen, D.S., “Acclimation of anaerobic sludge degrading chlorophenols and the biodegradation kinetics during acclimation period”, Chemosphere, 54, 1573-1580 (2004).
9 Ruzgas, T., Emneus, J., Gorton, L., Marko, V.G., “The development of a peroxidase biosensor for monitoring phenol and related aromatic compounds”, Anal. Chem. Acta, 31, 245-253 ( 1995).
10 Yamada, K., Akiba, Y., Shibuya, T., Kashiwada, A., Matsuda, K., Hirata, M., “Water purification through bioconversion of phenol compounds by tyrosinase and chemical adsorption by chitosan beads”, Biotechnol. Prog., 21, 823-829 (2005).
11 Garibay-Orijel, C., Ríos-Leal, E., García-Mena, J., Poggi-Varaldo, H.M., “2,4,6-Trichlorophenol and phenol removal in methanogenic and partially aerated methanogenic conditions in a fluidized bed bioreactor”, J. Chem. Technol. Biotechnol., 80, 1180-1187 (2005).
12 Garibay-Orijel, C., Hoyo-Vadillo, C., Ponce-Noyola, M.T., García-Mena, J., Poggi-Varaldo, H.M., “Impact of long-term partial aeration on the removal of 2,4,6-trichlorophenol in an initially methanogenic fluidized bed bioreactor”, Biotechnol. Bioeng., 94, 949-960 (2006). 13 Kargi, F., Eker, S., Uygur, A., “Biological treatment of synthetic wastewater containing 2,4-dichlorophenol (DCP) in an activated sludge unit”, J. Environ. Manage., 76, 191-196 (2005).
14 Bolanos, R.M.L., Damianovic, M.H.R.Z., Zaiat, M., Foresti, E., “Assessment of the ability of sludge to degrade PCP under anaerobic conditions”, Braz. J. Chem. Eng., 22, 611-617 (2005).
15 Wase, D.A.J., Hough, J.S., “Continuous culture of yeast on phenol”, J. Gen. Microbiol., 42, 13-23 (1966).
16 M rsen, A., Rehm, H.J., “Degradation of phenol by a defined mixed culture immobilized by adsorption on activated carbon and sintered glass”, Appl. Microbiol. Biotechnol., 33, 206-212 (1990).
17 Denizli, A., Cihangir, N., Rad, A.Y., Taner, M., Alsancak, G., “Removal of chlorophenols from synthetic solutions using Phanerochaete chrysosporium”, Process Biochem., 39, 2025-2030 (2004).
18 Kai-Chee, L., Sudhir, R., “External-loop fluidized bed airlift bioreactor (EFBAB) for the cometabolic biotransformation of 4-chlorophenol (4-cp) in the presence of phenol”, Chem. Eng. Sci., 60, 6313-6319 (2005).
19 Fialová, A., Boschke, E., Bley, T., “Rapid monitoring of the biodegradation of phenol-like compounds by the yeast Candida maltosa using BOD measurements”, Int. Biodeter. Biodegr., 54, 69-76 (2004).
20 Zouari, H., Labat, M., Sayadi, S., “Degradation of 4-chlorophenol by the white rot fungus Phanerochaete chrysosporium in free and immobilized cultures”, Bioresour. Technol., 84, 145-150 (2002).
21 Ivoilov, V.S., Karasevich, I.N., “Monochlorophenols as enzymes substrates for the preparatory metabolism of phenol in Candida tropicalis yeasts”, Mikrobiologiia, 52, 956-961 (1983).
22 Krug, M., Ziegler, H., Straube, G., “Degradation of phenolic compounds by the yeast Candida tropicalis HP 15.I, physiology of growth and substrate utilization”, J. Basic Microbiol., 25, 103-110 (1985).
23 Kim, S.B., “Isolation and characterization of a phenol-degrading Candida tropicalis PW-51”, Korean J. Appl. Microbiol. Biotechnol., 24, 743-748 (1996).
24 Jiang, Y., Wen, J.P., Li, H.M., Yang, S.L., Hu, Z.D., “The biodegradation of phenol at high initial concentration by the yeast Candida tropiclis”, Biochem. Eng. J., 24, 243-247 (2005).
25 Jiang, Y., Wen, J.P., Jia, X.Q., Canyin, Q., Hu, Z.D., “Mutation of Candida tropicalis by irradiation with a He-Ne laser to increase its ability to degrade phenol”, Appl. Environ. Microbiol., 73, 226-231 (2007).
26 Haydée, T.G., “Ploidy study in Sporothrix schenkii”, Fungal Genet. Biol., 27, 49-54 (1999).
27 Zeng, F., Cui, K.Y., Fu, J.M., Sheng, G.Y., Yang, H.F., “Biodegradability of di (2-ethylhexyl) phthalate by Pseudomonas fluorescens FS1”, Water Air Soil Poll., 140, 297-305 (2002).
28 Andrews, J.F., “A mathematical model for continuous culture of microorganisms utilizing inhibitory substrates”, Biotechnol. Bioeng., 10, 707-723 (1968).

[1]	Masoumeh Sheikh Hosseini Lori, Mohammad Delnavaz, Hoda Khoshvaght. Synthesizing and characterizing the magnetic EDTA/chitosan/CeZnO nanocomposite for simultaneous treating of chromium and phenol in an aqueous solution[J]. 中国化学工程学报, 2023, 58(6): 76-88.
[2]	Chaobo Zhang, Xiaoyong Yang, Jian Dai, Wenxia Liu, Hang Yang, Zhishan Bai. Efficient extraction of phenol from wastewater by ionic micro-emulsion method: Anionic and cationic[J]. 中国化学工程学报, 2023, 58(6): 137-145.
[3]	Bin Gao, Junwen Chen, Qi Zuo, Hongyan Wang, Wenlin Li. The critical role of Zr in controlling the activity of Pd/Beta on the hydrogenation of phenol to cyclohexanone[J]. 中国化学工程学报, 2023, 57(5): 79-87.
[4]	Chao Zhang, Youzhi Liu, Weizhou Jiao, Hongyan Shen, Xigang Yuan, Shengkun Jia. An optimization method for enhancement of gas–liquid mass transfer in a bubble column reactor based on the entropy generation extremum principle[J]. 中国化学工程学报, 2023, 53(1): 83-88.
[5]	Jian Tian, Gen Li, Wang He, Kok Bing Tan, Daohua Sun, Junfu Wei, Qingbiao Li. Insight into the dynamic adsorption behavior of graphene oxide multichannel architecture toward contaminants[J]. 中国化学工程学报, 2023, 53(1): 124-132.
[6]	Lijian Shi, Yaping Zhang, Yujia Tong, Wenlong Ding, Weixing Li. Plant-inspired biomimetic hybrid PVDF membrane co-deposited by tea polyphenols and 3-amino-propyl-triethoxysilane for high-efficiency oil-in-water emulsion separation[J]. 中国化学工程学报, 2023, 53(1): 170-180.
[7]	Lu Lv, Min Zhao, Yanan Liu, Yufei He, Dianqing Li. Fabrication of hydrophobic Pd/Al₂O₃-phosphoric acid via P-O-Al bond for liquid hydrogenation reaction[J]. 中国化学工程学报, 2023, 53(1): 232-242.
[8]	Kechang Gao, Shengjuan Shao, Zhixing Li, Jiaxin Jing, Weizhou Jiao, Youzhi Liu. Kinetics of the direct reaction between ozone and phenol by high-gravity intensified heterogeneous catalytic ozonation[J]. 中国化学工程学报, 2023, 53(1): 317-323.
[9]	Xinyu Lu, Dandan Wang, Haoquan Guo, Pengcheng Xiu, Jiajia Chen, Yu Qin, Hossain Mahmud Robin, Chaozhong Xu, Xingguang Zhang, Xiaoli Gu. Insights into depolymerization pathways and mechanism of alkali lignin over a Ni_1.2–ZrO₂/WO₃/γ-Al₂O₃ catalyst[J]. 中国化学工程学报, 2022, 48(8): 191-201.
[10]	Chunhua Zhang, Zhengyan Qu, Hong Jiang, Rizhi Chen, Weihong Xing. Nb₂O₅ promoted Pd/AC catalyst for selective phenol hydrogenation to cyclohexanone[J]. 中国化学工程学报, 2022, 44(4): 87-93.
[11]	Shaimaa T. Kadhum, Ghayda Yassen Alkindi, Talib M. Albayati. Eco friendly adsorbents for removal of phenol from aqueous solution employing nanoparticle zero-valent iron synthesized from modified green tea bio-waste and supported on silty clay[J]. 中国化学工程学报, 2021, 36(8): 19-28.
[12]	Shuai Xu, Qi Zhu, Shaojie Xu, Manjing Yuan, Xuliang Lin, Wenjing Lin, Yanlin Qin, Yuliang Li. The phase behavior of n-ethylpyridinium tetrafluoroborate and sodium-based salts ATPS and its application in 2-chlorophenol extraction[J]. 中国化学工程学报, 2021, 33(5): 76-82.
[13]	Zhenming Zhang, He Zhao, Xiaohui Wang, Weiming Ni, Fengsheng Gao, Jianrong Wang, Minjin Liu, Yongli Li. HPLC and GC methods development for the analysis of key intermediate for synthesis of dicamba[J]. 中国化学工程学报, 2021, 33(5): 112-117.
[14]	Xinlong Yan, Yanfang Li, Xiaoyan Hu, Rui Feng, Min Zhou, Dezhi Han. Enhanced adsorption of phenol from aqueous solution by carbonized trace ZIF-8-decorated activated carbon pellets[J]. 中国化学工程学报, 2021, 33(5): 279-285.
[15]	Xueping Liu, Ping Xue, Feng Jia, Dongya Qiu, Keren Shi, Weiwei Zhang. Tailoring polymeric composite gel beads-encapsulated microorganism for efficient degradation of phenolic compounds[J]. 中国化学工程学报, 2021, 32(4): 301-306.

Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2

Biodegradation of Phenol and 4-Chlorophenol by the Mutant Strain CTM 2

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价