Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

doi:10.1016/j.cjche.2014.09.011

›› 2014, Vol. 22 ›› Issue (11/12): 1215-1219.DOI: 10.1016/j.cjche.2014.09.011

• 催化、动力学与反应工程 • 上一篇下一篇

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Junfeng Liu¹, Li Deng^1,2, Meng Wang¹, Kaili Nie^1,2, Luo Liu¹, Tianwei Tan¹, Fang Wang^1,3

1 Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China;
2 Amoy-BUCT Industrial of Bio-technovation Institute, Amoy 361022, China;
3 State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

收稿日期:2013-01-05 修回日期:2013-08-19 出版日期:2014-12-28 发布日期:2014-12-24
通讯作者: Li Deng
基金资助:
Supported by Key Projects in the National Science & Technology Pillar Programduring the 12th Five-year Plan Period (2011BAD22B04), the National Basic Research Program of China (973 program) (2011CB710800, 2011CB200905, 2009CB724703), the National Natural Science Foundation of China (21076017), the National High-Tech R&D Program of China (863 Program) (2012AA021404, 2012AA021402), the Fundamental Research Funds for the Central Universities (ZY1331).

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Junfeng Liu¹, Li Deng^1,2, Meng Wang¹, Kaili Nie^1,2, Luo Liu¹, Tianwei Tan¹, Fang Wang^1,3

1 Beijing Key Laboratory of Bioprocess, Beijing University of Chemical Technology, Beijing 100029, China;
2 Amoy-BUCT Industrial of Bio-technovation Institute, Amoy 361022, China;
3 State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2013-01-05 Revised:2013-08-19 Online:2014-12-28 Published:2014-12-24
Supported by:
Supported by Key Projects in the National Science & Technology Pillar Programduring the 12th Five-year Plan Period (2011BAD22B04), the National Basic Research Program of China (973 program) (2011CB710800, 2011CB200905, 2009CB724703), the National Natural Science Foundation of China (21076017), the National High-Tech R&D Program of China (863 Program) (2012AA021404, 2012AA021402), the Fundamental Research Funds for the Central Universities (ZY1331).

摘要/Abstract

摘要： The non-edible camphor tree seed oil was extracted and catalyzed by immobilized lipase for biodiesel production. The oil yield from camphor tree seeds reached 35.2% of seed weight by twice microwave-assisted extractions. Gas chromatography showed that free fatty acid content in camphor tree seed oil was 1.88%, and the main fatty acids were capric acid (53.4%) and lauric acid (38.7%).With immobilized lipase Candida sp. 99-125 as catalyst, several important factors for reaction conditions were examined through orthogonal experiments. The optimum conditions were obtained: water content and enzyme loading were both 15% with a molar ratio of 1:3.5 (oil/ethanol), and the process of alcoholysis was in nine steps at 40 ℃ for 24 h, with agitation at 170 r·min^-1. As a result, the medium-chain biodiesel yield was 93.5%. The immobilized lipase was stable when it was used repeatedly for 210 h.

关键词: Camphor tree seed oil, Medium-chain biodiesel, Lipase, Ethanolysis

Abstract: The non-edible camphor tree seed oil was extracted and catalyzed by immobilized lipase for biodiesel production. The oil yield from camphor tree seeds reached 35.2% of seed weight by twice microwave-assisted extractions. Gas chromatography showed that free fatty acid content in camphor tree seed oil was 1.88%, and the main fatty acids were capric acid (53.4%) and lauric acid (38.7%).With immobilized lipase Candida sp. 99-125 as catalyst, several important factors for reaction conditions were examined through orthogonal experiments. The optimum conditions were obtained: water content and enzyme loading were both 15% with a molar ratio of 1:3.5 (oil/ethanol), and the process of alcoholysis was in nine steps at 40 ℃ for 24 h, with agitation at 170 r·min^-1. As a result, the medium-chain biodiesel yield was 93.5%. The immobilized lipase was stable when it was used repeatedly for 210 h.

Key words: Camphor tree seed oil, Medium-chain biodiesel, Lipase, Ethanolysis

Junfeng Liu, Li Deng, Meng Wang, Kaili Nie, Luo Liu, Tianwei Tan, Fang Wang. Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil[J]. , 2014, 22(11/12): 1215-1219.

参考文献

[1] H. Tian, C. Li, C. Yang, H. Shan, Alternative processing technology for converting vegetable oils and animal fats to clean fuels and light olefins, Chin. J. Chem. Eng. 16 (3) (2008) 394-400.
[2] A. Srivastava, R. Prasad, Triglycerides-based diesel fuels, Renew. Sust. Energ. Rev. 4 (2) (2000) 111-133.
[3] Y. Liu, H. Lu,W. Jiang, D. Li, S. Liu, B. Liang, Biodiesel production from crude Jatropha curcas L. oil with trace acid catalyst, Chin. J. Chem. Eng. 20 (4) (2012) 740-746.
[4] K.R. Jegannathan, S. Abang, D. Poncelet, E.S. Chan, P. Ravindra, Production of biodiesel using immobilized lipase—a critical review, Crit. Rev. Biotechnol. 28 (4) (2008) 253-264 (2008).
[5] S.C. Tupufia, Y.J. Jeon, C. Marquis, A.A. Adesina, P.L. Rogers, Enzymatic conversion of coconut oil for biodiesel production, Fuel Process. Technol. 106 (2013) 721-726.
[6] Z. Jin, S.Y. Han, L. Zhang, S.P. Zheng, Y.Wang, Y. Lin, Combined utilization of lipasedisplaying Pichia pastoris whole-cell biocatalysts to improve biodiesel production in co-solvent media, Bioresour. Technol. 130 (2013) 102-109.
[7] Y.Y. Shi, Preparation light biodiesel from camphor tree seed oil, Master Thesis, Nanchang Univ., China, 2008. (in Chinese).
[8] Y. Chen, J. Chen, Y. Luo, Complementary biodiesel combination from tung and medium-chain fatty acid oils, Renew. Energy 44 (2012) 305-310.
[9] K. Gerhard, C.C. Steven, L.E. Roque, Cuphea oil as source of biodiesel with improved fuel properties caused by high content of methyl decanoate, Energy Fuels 23 (2009) 1743-1747.
[10] A. Hayyan, F.S. Mjalli, M.A. Hashim, M. Hayyan, I.M. AlNashef, S.M. Al-Zahrani, M.A. Al-Saadi, Ethanesulfonic acid-based esterification of industrial acidic crude palm oil for biodiesel production, Bioresour. Technol. 102 (20) (2011) 9564-9570.
[11] S.X. Zhao, Borneol — the world's first applicated drug of natural and organic ingredients, Asia Pac. Tradit. Med. 2 (2006) 24-25.
[12] R.Y. Yeh, Y.L. Shiu, S.C. Shei, S.C. Cheng, S.Y. Huang, J.C. Lin, C.H. Liu, Evaluation of the antibacterial activity of leaf and twig extracts of stout camphor tree, Cinnamomum kanehirae, and the effects on immunity and disease resistance of white shrimp, Litopenaeus vannamei, Fish Shellfish Immunol 27 (1) (2009) 26-32.
[13] W. Zhou, B. Zhang, D. Deng, Oil extraction fromcamphor tree seed, China Oils Fats 29 (2) (2004) 30-31.
[14] Z. Zeng, Y. Shi, L. Liang, C. Peng, Esterification and transesterification of camphor tree seed oil continuously catalyzed by concentrated sulphuric acid, J. Nanchang Univ. (Nat. Sci. Ed.) 32 (6) (2008) 575-579.
[15] K. Nie, F. Xue, F. Wang, T. Tan, Lipase catalyzed methanolysis to produce biodiesel: optimization of the biodiesel production, J. Mol. Catal. B Enzym. 43 (1) (2006) 142-147.
[16] Z. Li, L. Deng, J. Lu, X. Guo, Z. Yang, T. Tan, Enzymatic synthesis of fatty acid methyl esters from crude rice bran oil with immobilized Candida sp. 99-125, Chin. J. Chem. Eng. 18 (5) (2010) 870-875.
[17] J. Lu, K. Nie, F. Xue, F. Wang, T. Tan, Enzymatic synthesis of fatty acid methyl esters from lard with immobilized Candida sp. 99-125, Process Biochem. 42 (9) (2007) 1367-1370.
[18] J. Lu, Y. Chen, F. Wang, T. Tan, Effect of water on methanolysis of glycerol trioleate catalyzed by immobilized lipase Candida sp. 99-125 in organic solvent system, J. Mol. Catal. B Enzym. 56 (2) (2009) 122-125.
[19] C. Yin, Microwave-assisted pyrolysis of biomass for liquid biofuels production, Bioresour. Technol. 120 (2012) 273-284.
[20] J.Wang, Y.Wang, L. Zheng, S. Ni, Z. Fan, R. Yao, K. Chen, Kinetic study on extraction of red pepper seed oil with supercritical CO₂, Chin. J. Chem. Eng. 22 (1) (2014) 44-50.
[21] J. Sun, B. Yu, P. Curran, S. Liu, Lipase-catalysed transesterification of coconut oil with fusel alcohols in a solvent-free system, Food Chem. 134 (1) (2012) 89-94.
[22] T. Tan, J. Lu, K. Nie, L. Deng, F.Wang, Biodiesel production with immobilized lipase: a review, Biotechnol. Adv. 28 (5) (2010) 628-634.
[23] P. Lv, X. Wang, Z. Yuan, T. Tan, Conversion of soybean oil to biodiesel fuel with immobilized Candida lipase on textile cloth, Energy Sources Part A 30 (9) (2008) 872-879.
[24] T. Tan, K. Nie, F.Wang, Production of biodiesel by immobilized Candida sp. lipase at high water content, Appl. Biochem. Biotechnol. 128 (2) (2006) 109-116.
[25] J. Hou, J. Lu, K. Nie, F.Wang, T. Tan, Synthesis of fatty acid ethyl esters from soybean oil by immobilized lipase from Candida sp. 99-125, Chin. J. Process. Eng. 8 (2) (2008) 355-358.
[26] V. Dossat, D. Combes, A.Marty, Lipase-catalysed transesterification of high oleic sunflower oil, Enzyme Microb. Technol. 30 (2002) 90-94.
[27] M.M. Soumanou, U.T. Bornscheuer, Improvement in lipase-catalyzed synthesis of fatty acid methyl esters from sunflower oil, Enzyme Microb. Technol. 33 (1) (2003) 97-103.
[28] G. Chen, M. Ying,W. Li, Enzymatic conversion of waste cooking oils into alternative fuel-biodiesel, Appl. Biochem. Biotechnol. 132 (2006) 911-921.
[29] Y.Wang, H.Wu, M. Zong, Improvement of biodiesel production by lipozyme TL IMcatalyzedmethanolysis using response surfacemethodology and acylmigration enhancer, Bioresour. Technol. 99 (2008) 7232-7237.
[30] S.K. Hoekman, B. Amber, R. Curtis, C. Eric, N. Mani, Review of biodiesel fatty composition, properties, and specifications, Renew. Sust. Energ. Rev. 16 (2012) 143-169.

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

Lipase Catalyzed Synthesis of Medium-chain Biodiesel from Cinnamonum camphora Seed Oil

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	Pengcheng Zou, Kai Wang. Methanolysis of amides under high-temperature and high-pressure conditions with a continuous tubular reactor[J]. 中国化学工程学报, 2023, 58(6): 170-178.
[2]	Chunyu Zhang, Yan Sun, Xiaoyan Dong. Conjugation of a zwitterionic polymer with dimethyl chains to lipase significantly increases the enzyme activity and stability[J]. 中国化学工程学报, 2022, 47(7): 48-53.
[3]	Saboura Ashkevarian, Jalil Badraghi, Fatemeh Mamashli, Behdad Delavari, Ali Akbar Saboury. Covalent immobilization and characterization of Rhizopus oryzae lipase on core-shell cobalt ferrite nanoparticles for biodiesel production[J]. 中国化学工程学报, 2021, 37(9): 128-136.
[4]	Ning Chen, Chunyu Zhang, Xiaoyan Dong, Yan Sun. Fabrication and characterization of epoxylated zwitterionic copolymergrafted silica nanoparticle as a new support for lipase immobilization[J]. 中国化学工程学报, 2020, 28(4): 1129-1135.
[5]	Yifeng Li, Chunyu Zhang, Yan Sun. Zwitterionic polymer-coated porous poly(vinyl acetate-divinyl benzene) microsphere: A new support for enhanced performance of immobilized lipase[J]. 中国化学工程学报, 2020, 28(1): 242-248.
[6]	Zhuhua Chan, RunpingWang, Fan Yang, Runying Zeng. Enhanced cold active lipase production by metagenomic library recombinant clone CALIP3 with a step-wise temperature and dissolved oxygen level control strategy[J]. , 2016, 24(9): 1263-1269.
[7]	Ya Li, Jicheng Hu, Pingfang Han. Synthesis of magnetically modified palygorskite composite for immobilization of Candida sp. 99-125 lipase via adsorption[J]. Chinese Journal of Chemical Engineering, 2015, 23(5): 822-826.
[8]	Hongli Yang, YingMu, Hongtao Chen, Chunyang Su, Tiankui Yang, Zhilong Xiu. Sn-1,3-specific Interesterification of Soybean Oil with Medium-chain Triacylglycerol Catalyzed by Lipozyme TL IM[J]. , 2014, 22(9): 1016-1020.
[9]	Jingyun Wang, Fangling Ji, Jishuang Xing, Shuang Cui, Yongming Bao, Wenbo Hao . Lipase Immobilization onto the Surface of PGMA-b-PDMAEMA-grafted Magnetic Nanoparticles Prepared via Atom Transfer Radical Polymerization[J]. , 2014, 22(11/12): 1333-1339.
[10]	王俊, 李晶, 张磊霞, 顾双双, 吴福安. Lipase-catalyzed Synthesis of Caffeic Acid Phenethyl Ester in Ionic Liquids：Effect of Specific Ions and Reaction Parameters[J]. Chinese Journal of Chemical Engineering, 2013, 21(12): 1376-1385.
[11]	邓利, 王晓静, 聂开立, 王芳, 刘军峰, 王璞, 谭天伟. Synthesis of Wax Esters by Lipase-catalyzed Esterification with Immobilized Lipase from Candida sp. 99-125[J]. , 2011, 19(6): 978-982.
[12]	尹春华, 张聪, 高明. Enzyme-catalyzed Synthesis of Vitamin E Succinate Using a Chemically Modified Novozym-435[J]. , 2011, 19(1): 135-139.
[13]	李政, 邓利, 鲁吉珂, 郭小雷, 杨自信, 谭天伟. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. , 2010, 18(5): 870-875.
[14]	YINChunhua(尹春华),LIUTao(刘涛)andTANTianwei(谭天伟). Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media[J]. , 2006, 14(1): 81-86.
[15]	宋宝东, 丁辉, 吴金川, Hayashi Y., Talukder MMR, 王世昌. 表面活性剂包衣Candida rugosa脂肪酶在无溶剂下油水两相体系中催化橄榄油水解[J]. , 2003, 11(5): 601-603.