Mining and engineering of terpene synthases and their applications in biomanufacturing

doi:10.1016/j.cjche.2025.08.001

中国化学工程学报 ›› 2025, Vol. 86 ›› Issue (10): 138-149.DOI: 10.1016/j.cjche.2025.08.001

• Special Issue on Celebrating the 100th Anniversary of the School of Chemical Engineering and Technology of Tianjin University • 上一篇下一篇

Mining and engineering of terpene synthases and their applications in biomanufacturing

Yue Xu¹, Chenwen Liu², Lei Qin^2,3,4,5,6, Bo Lv⁷, Genlin Zhang¹, Chun Li^2,3,4,5,6,7

1. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;
2. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
3. Key Lab for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing 100084, China;
4. Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;
5. Beijing Key Laboratory of Recombinant Protein Synthetic Biomanufacturing, Beijing 100084, China;
6. State Key Laboratory of Green Biomanufacturing, Beijing 100084, China;
7. Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China

收稿日期:2025-03-30 修回日期:2025-05-17 接受日期:2025-08-01 出版日期:2025-10-28 发布日期:2025-08-07
通讯作者: Genlin Zhang,E-mail:zhgl_food@sina.com;Chun Li,E-mail:lichun@tsinghua.edu.cn
基金资助:
This work was supported by the National Key Research and Development Program of China (2020YFA0908300) and the Natural Science Foundation of China (22138006, 22278240).

Mining and engineering of terpene synthases and their applications in biomanufacturing

Yue Xu¹, Chenwen Liu², Lei Qin^2,3,4,5,6, Bo Lv⁷, Genlin Zhang¹, Chun Li^2,3,4,5,6,7

1. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832003, China;
2. Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;
3. Key Lab for Industrial Biocatalysis, Ministry of Education, Tsinghua University, Beijing 100084, China;
4. Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China;
5. Beijing Key Laboratory of Recombinant Protein Synthetic Biomanufacturing, Beijing 100084, China;
6. State Key Laboratory of Green Biomanufacturing, Beijing 100084, China;
7. Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China

Received:2025-03-30 Revised:2025-05-17 Accepted:2025-08-01 Online:2025-10-28 Published:2025-08-07
Contact: Genlin Zhang,E-mail:zhgl_food@sina.com;Chun Li,E-mail:lichun@tsinghua.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2020YFA0908300) and the Natural Science Foundation of China (22138006, 22278240).

摘要/Abstract

摘要： Terpenoids, one of the most diverse and structurally varied natural products in nature, are widely distributed in plants, microbes, and other organisms. Their structural diversity confers significant importance in medicine, food, flavorings, and energy. However, traditional methods of plant extraction and chemical synthesis have limitations in industrial applications. Consequently, microbial cell factories have emerged as an important platform for terpenoid production. Terpene synthases (TPSs) are crucial in determining the structural and functional diversity of terpenoids. This review discussed the origin and classification of TPSs, outlines commonly used TPS mining methods, and summarizes advances in TPS engineering. In addition, it also explores the influence of machine learning on enzyme mining, the existing challenges and the future opportunities alongside cutting-edge technologies.

关键词: Biocatalysis, Enzymes, Synthetic biology, Terpene synthases, Enzyme mining, Enzyme engineering

Abstract: Terpenoids, one of the most diverse and structurally varied natural products in nature, are widely distributed in plants, microbes, and other organisms. Their structural diversity confers significant importance in medicine, food, flavorings, and energy. However, traditional methods of plant extraction and chemical synthesis have limitations in industrial applications. Consequently, microbial cell factories have emerged as an important platform for terpenoid production. Terpene synthases (TPSs) are crucial in determining the structural and functional diversity of terpenoids. This review discussed the origin and classification of TPSs, outlines commonly used TPS mining methods, and summarizes advances in TPS engineering. In addition, it also explores the influence of machine learning on enzyme mining, the existing challenges and the future opportunities alongside cutting-edge technologies.

Key words: Biocatalysis, Enzymes, Synthetic biology, Terpene synthases, Enzyme mining, Enzyme engineering

Yue Xu, Chenwen Liu, Lei Qin, Bo Lv, Genlin Zhang, Chun Li. Mining and engineering of terpene synthases and their applications in biomanufacturing[J]. 中国化学工程学报, 2025, 86(10): 138-149.

Yue Xu, Chenwen Liu, Lei Qin, Bo Lv, Genlin Zhang, Chun Li. Mining and engineering of terpene synthases and their applications in biomanufacturing[J]. Chinese Journal of Chemical Engineering, 2025, 86(10): 138-149.

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Mining and engineering of terpene synthases and their applications in biomanufacturing

Mining and engineering of terpene synthases and their applications in biomanufacturing

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